0 Investigation of Octopus rubescens Population Dynamics: Population Fluctuations at 200m Depth in Monterey Bay Kristin B. Hulvey Advisors: Charles Baxter and James Watanabe Hopkins Marine Station Stanford University Spring Quarter 1997 Permission is granted to Stanford University to use the citation and abstract of this paper. Permanent address Hopkins Marine Station Stanford University Pacific Grove, CA 93950 Abstract In October of 1991, a National Marine Fishery Survey conducted at the 200 m depth contour along a segment of sand-mud canyon bottom between Ascension Canyon and Monterey Canyon in the Monterey Bay revealed an unusually large population of Octopus rubescens (852 octopuses ha, SE=179.8). When re-sampled in March of 1997 using a remotely operated vehicle (ROV), the O. rubescens population was found to have significantly decreased in size (3 octopuses ha", SE-3.2, p«0.001). Fishery effects on predatory rockfish and factors affecting juvenile recruitment were found to be the most plausible causes for the large 1991 O. rubescens populations. The effect of these factors could not be established for the smaller 1997 populations because of insufficient data. Large increases in numbers of harbor seals, Phoca vitulina, within the bay may be contributing significantly to the O. rubescens decline. Introduction The factors leading to population fluctuations in the deep sea benthic communities of Monterey Bay are not clearly understood. Without a knowledge of these factors, human impacts on marine ecosystems cannot fully be quantified. In 1991, a National Marine Fisheries Survey discovered a large population of Octopus rubescens (one every 0.8 m), along a section of sand-mud canyon floor at 200 m depth (Charles Baxter, per. com.). Such an unusual population provided an unique opportunity to investigate factors which may be having obvious effects on a population sizes of a species in the Monterey Bay. O. rubescens is found from Alaska to Baja California to the Gulf of California (Hochberg and Fields, 1980). The life span of the O. rubescens ranges from one (Hochberg and Fields, 1980) to two years (Dorsey, 1976), with juveniles spending an unknown amount of time in a pelagic phase. While in the water column, juveniles stay at depths of 400 m or less (Hunt, 1996), and feed mainly on zooplankton (Dorsey, 1976). Adult O. rubescens inhabit a variety of habitats ranging from shallow water kelp beds to sand-mud canyon bottoms at up to 250 m depth, and feed mainly on crustaceans, mollusks, and fishes (Hochberg and Fields, 1980). The goals of this research were to both determine how the population of O. rubescens along the transect had changed from 1991 to 1997, as well as to investigate some of the factors which may be causing changes in populations of the O. rubescens. Due to the history of commercial fishing in the area of the transect, as well as the life history of the O. rubescens, fishery activity and factors affecting O. rubescens survival during their time in the pelagic phase were both investigated as possible causes of population fluctuation. Additionally, because of increases in numbers of harbor seals in the area, predation pressure from seals was also investigated. Methods Study Site The study site is located on a section of sand-mud ocean bottom at 200 m depth between Monterey and Ascension canyons (Fig. 1). For a detailed description of the transect area see Adams et al. (1995). The transect began at N 36° 47’ latitude W 122° 06' longitude and extended for approximately 10 km, ending at approximately N 36° 48' latitude W 122° 08' longitude. Transect Sampling The 1991 transect was re-sampled on March 21, 1997 using the Monterey Bay Aquarium Research Institute’s (MBARI) remotely operated vehicle (ROV), Ventana. ROV location was measured every few seconds using a combined navigational system consisting of a differential global positioning system and a sonar system. RÖV depth and altitude off substrate were measured simultaneously with location and were recorded to the nearest meter. Using telemetry as a guide, the path of the ROV tracked the 200 m contour as closely as possible. The average height above the substrate was 0.87 m, and average speed of the ROV varied because of strong tail currents. For detailed descriptions of the ROV camera specifications see Adams et al., (1995 Video Analysis Video tapes of both the 1991 and 1997 transects were viewed at least three times to identify organisms found along the transect. All visible organisms on the video tape were identified to the lowest taxonomic level, and their abundances were recorded. O. rubescens were sub-sampled in order to quantify the large change in population numbers observed from 1991 to 1997. Rockfishes were similarly sub-sampled due to possible biological interactions between octopus and rockfish populations. To obtain sub-sampled areas, the 1991 and 1997 transects were divided into 52 and 93 segments, respectively, with each segment being as close to 100 m as possible. The length of each segment was determined from the difference between successive location estimates. Ten segments were randomly chosen from each transect sample, and octopus and rockfish numbers were counted and recorded. Total transect area was calculated by multiplying transect width by transect length. Transect length was determined by summing distances between successive Northing and Easting measurements. A total of 5984.1 m of 1991 transect and 9154.1 m of 1997 transect was used in the analysis. The width of the 1991 transect was 1.8 m (Adams et al., 1995). The width of the 1997 transect, 3.1 m, was calculated by using the constant distance between two laser points (28 cm) and extrapolating the transect width from the total monitor width. Because the laser system was only operating at the end of the transect run, the width calculated from the lasers during this time was assumed to be the same as the average width of the whole transect. 1o account for differing total areas seen in 1991 and 1997, all density estimates were normalized to number of organism per hectare (10,000 m2). Portions of the video tape from 1991 and 1997 were unusable and omitted from transect analysis. Reasons for omission included: the ROV was too far above the substrate for organisms to be counted and identified, the ROV was sitting on the bottom motionless, the ROV wandered + 20 m off of the 200 m contour, or the recorded length of a segment between two time points was too large to be reasonable. A G-test of independence (Sokal and Rohlf, 1981) was used to test for changes in relative abundance occurring from 1991 to 1997. Additionally the Shannon Index was used as a measure of species diversity (-2p, In(p), where p=proportion of the i“ species; Magurran, 1988). Student's t-tests were performed on sub-sampled data in order to establish if octopus and rockfish numbers had changed significantly between 1991 and 1997. Octopus variances were significantly different (F-test, P20.01), requiring the use of an unequal variance t-test. Rockfish data were log transformed resulting in homogenous variances (F-test, P-0.28), and t-tests were performed on these transformed data. Fishery Data Fishery data from 1980 to 1986 were obtained from the California Department of Fish and Game. To monitor fish landings, the Department of Fish & Game has divided the Monterey Bay into a series of 18 km by 18 km quadrats. Fishing activity is monitored in each quadrat, and type of fishing, depth of fishing (if trawling is used), length of fishing run, and pounds of each species or market group caught are recorded. For this study, data of rockfish taken by otter trawl along the 200 m + 20 m depth contour from Fish & Game block 518 were used. Most of the transect fell within this quadrat area, although the 200 m + 20 m depth contour within block 518 was about twice as long as the total transect length. Pounds of rockfish taken per year (hereafter referred to as "take") were used as an indicator of fishing stress on the rockfish population, while pounds of rockfish per hour of fishing time was used as a rough indicator of rockfish abundance. It was assumed that changes in take and abundance along the whole 200 m + 20 m depth contour in block 518 fluctuated in the same manner as take and abundance along the length of the transect. Juvenile Octopus Data Numbers of juveniles in the water column were calculated from mid-water transect videos obtained from MBARI's video database. All mid-water transects occurred at a regularly sampled site at the axis of the Monterey Canyon, and spanned the years of 1989 to 1995. A search of the general database, which consisted of video through 1994, resulted in 40 transects where octopuses were recorded. A second database consisting mainly of 1995 video was searched, and an additional 9 transects were identified as having octopuses. Sections of video annotated as having sightings of single octopuses were not examined, and a single octopus was recorded to have been seen. Sections of video annotated as having sightings of octopus populations were viewed and the number of octopuses seen were recorded. Quantifying numbers of juveniles became much more difficult if swarms of octopus were spotted. Swarms of octopuses often faded into the distance, with individuals fading in and out of focus. Additionally, because swarms often covered an area larger than the camera’s field of view, individuals swimming off screen could swim back through a moment later and be counted more than once. In order to standardize how groups of octopuses were counted, a grading system was employed. A population score of 5 was given to any swarm where 5 to 9 individuals could be seen in the video frame at any given moment. A population score of 10 was given for to swarms of 10 to 14 individuals, and a score of 15 was given to swarms of 15 or greater. For groups of less than 5 individuals, the exact number present was determined. No swarms greater than 20 octopuses per frame were observed. Total numbers of juveniles seen per year were divided by total numbers of dive hours for that year. Dive hours were estimated to the nearest half an hour and included videos where no octopuses were seen. The resulting index was a measure of juvenile octopus abundance per year. Chlorophyll Data Mean chlorophyll levels from 1989 to 1996 were used as an indicator of mean phytoplankton abundance in the upper portion of the water column. Average yearly measurements of chlorophyll levels (ug 1*) occurring at 0 depth in the water column were calculated using chlorophyll measurements obtained from MBARI (Chavez, unpubl. data). It was assumed that levels of chlorophyll, and thus phytoplankton abundance at the surface, reflected general food availability for juvenile octopuses. From these data, regression relationships between chlorophyll and juvenile octopus abundances were estimated using time lags of zero, one, and two years. This relationship was used to establish relationships between fluctuations in standing crop of phytoplankton, and thus possibly zooplankton abundance, and fluctuations in juvenile octopus numbers. Harbor Seal Data Predation intensity by harbor seals was estimated from the occurrence of octopus beaks in seal scat. To estimate numbers of beaks in scats from the early 1990's, numbers of beaks found in seal scat taken from Elkhorn Slough from January 1991 to December 1991 (Oxman, pers. com.) were averaged with numbers of beaks found in Slough scats from May 1991 to May 1992 (Trumble, 1995). Estimates of numbers of beaks found in scats for the mid to late 1990's was determined from scat samples collected from September 1995 to September 1996 (Tomo Eguchi, unpubl. data). The 1995-1996 beak numbers were determined by counting beaks already extracted from scat samples obtained for a different study (Tomo Eguchi, unpubl data). The greatest number of either upper or lower beaks was used as the total number of beaks per scat. For both the early 90's and mid to late 90's, total beak numbers found in scat samples over the year were divided by total numbers of scats collected during the year to obtain an index of average beaks per scat per year. Results Transect Analysis Octopus rubescens decreased significantly from a mean of 852 ha“ in 1991 to 3 hal in 1997 (t-test, P«0.001, SE=179.8, 3.2, Fig. 2). An increase in body size was also noted among octopuses from the two samples, from approximately 5 cm in 1991 to 20 cm in 1997. Sizes were estimated as general size of the animal when sitting on the substrate and not strictly mantle size. Rockfish numbers increased significantly from a mean of 42 ha“ in 1991 to 54 ha“ in 1997 (t-test, P«.04, SE’s-32.3, 14.6, Fig. 3). Qualitative changes were also noted for body size of rockfishes. Most rockfishes along the transect in 1991 were approximately 20 to 40 cm total body length. The majority of rockfishes observed in 1997 were also in this 20 to 40 cm size class. However, there was an additional class of smaller rockfish, approximately 10 cm in length or smaller. Distribution of the rockfish population also changed from patchy in 1991, to more evenly spread along the transect in 1997. Relative abundances of other species found along the transect changed significantly (G.g=65.3, P«0.001). Octopus and rockfish populations were excluded from this calculation because their significance was already determined. Largest decreases in abundance from 1991 to 1997 included: the sea urchin Allocentrotus fragilis, slender sole (Eopsetta exilis), sand star (Luidia foliolata), and red sea star (Mediaster aequalis). Largest increases in abundances from 1991 to 1997 included: bigfin eelpout (Lycodes cortezianus), the sea star Rathbunaster californicus, and spotted cusk-eel (Chilara taylori) (Fig. 4). Species richness decreased from 27 in 1991 to 22 in 1997 and total diversity, as measured by the Shannon index, decreased significantly (H' for 1991 = 1.53, H' for 1997 = 1.02,t-7.41, P««0.001, Table 1) Fishery Data Rockfish take and abundance fluctuated from 1980 to 1996 between years of high take-high abundance and low take-low abundance (Fig. 5). Take is assumed to be a measure of stress inflicted on the rockfish population by the fishery because high take years are followed by low abundance years. It was assumed, therefore, that a high take could negatively affect the rockfish population by reducing it to a low level in subsequent years. A large take of approximately 45000 lb. of rockfish in 1990 was followed by a low abundance of 250 lb. of rockfish caught fishing hr’ in 1991. In 1995 and 1996 a take of approximately 25,000 lb. and 48,000 lb. of rockfish was coupled with abundances of approximately 300 and 400 lb. fishing hr". The large take in 1996 reflects a larger number of hours of fishing rather then a high abundance of rockfish. From the previously seen pattern of high take years being followed by low abundance years, a low abundance of rockfish is expected in 1997. Juvenile Octopus Data There was a general trend of decreasing numbers of juvenile octopuses in the water column from 1989 (=2.25 octopus hr*) to 1993 (=0.4 octopus hr*), with slight increases in numbers occurring in 1994 (20.41 octopus hr*) and 1995 (=0.08 octopus hr, Fig. 6). Chlorophyll abundance versus juvenile octopus abundance were plotted in three different ways. While all scatter plots had positive slopes, none had statistically significant correlations. This lack of statistical significance was probably due to the small number of points plotted as opposed to a lack of correlation, especially for the octopus abundance which was lagged by one year. For directly plotted abundances, the best fit line indicated that fluctuations in mean yearly chlorophyll accounted for only 1% of the fluctuations seen in octopus numbers (Fig. 7). For abundances plotted lagging octopus abundance one and two years behind chlorophyll abundance, resulting chlorophyll fluctuations explained 34% and 13% of the fluctuations seen in juvenile octopus abundance (Fig. 8 and 9). Harbor Seal Data The numbers of octopus beaks per scat sample decreased from approximately 5.5 during the early 1990’s to approximately 1.5 during the mid to late 1990's (n=547, 99, Fig. 10). Discussion Evidence suggests that fishing pressure, factors affecting the numbers of pelagic juvenile octopus recruiting to the benthic community, and increases in predation pressure by increasing numbers of harbor seals contributed to the significant fluctuations in Octopus rubescens populations between 1991 and 1997. Additionally, these or other factors may be affecting community-wide patterns of relative abundance and diversity. The study site is located in one of three most heavily fished areas in the Monterey Bay, with the 200m depth contour being the most heavily trawled depths in this site (Bob Leos, Dept. of F&G, per. com.). Rockfishes, a target species for trawl fisheries, use cephalopods as a major food source (Houk, 1992). Increases in rockfish numbers, from 46 ha“ in 1991 to 54 ha in 1997, accompanied by decreases in octopus numbers, from 853 ha’ to 3 ha", support the idea of a significant interaction between these two populations. Because of this, fishery activity along the transect area may be influencing octopus populations by affecting the numbers of predatory rockfish. It is not possible to determine the exact relationship between octopus abundance and fishery pressure. However, based on the assumption that rockfish take is affecting rockfish abundance and that rockfish abundance is affecting octopus abundance, the large rockfish take in 1990 followed by lower rockfish abundance in 1991 may have released octopuses from predation pressure in 1991. Such a release may have allowed octopus survival rates to be greater than normal along the transect, resulting in the larger population of octopuses found in 1991. It is harder to establish how fishing activity may have contributed to the greatly reduced octopus numbers seen in 1997. For reasons presented above (see results), fishery data predict a low rockfish abundance in 1997. Findings from video analysis, however, indicate that rockfish populations have increased significantly over the area of the transect in 1997. Although this result seems to contradict fishery predictions, it may be that the 1997 rockfish population, although decreasing from populations found in 1996, is still larger than populations found in 1991. Regardless, it is unlikely that the small increase in rockfish abundance, from 46 to 54 ha", fully explains the large decrease in octopus population found in 1997. More research is needed to establish connections between fishery activity and octopus population decreases. 13 A second factor that may have led to the large change in octopus numbers along the transect is a fluctuation in the numbers of juveniles recruiting to the benthic community. This hypothesis is supported by high abundances of octopuses in the two years immediately preceding the large population of benthic octopuses found in 1991 (Fig. 6). Because large abundances of juveniles were found in the two years preceding 1991, the data are consistent for both a one and two year O. rubescens life cycle. It is difficult to establish how recruitment class size affected 1997 benthic numbers because class size numbers for 1996 are not available. Juvenile abundance since 1989 generally decreased (Fig. 6), with small increases in both 1994 and 1995. If O. rubescens has a two year life cycle, the 1995 class may indicate the 1997 population size. Although juvenile abundance in 1995 was lower than in 1989 and 1990 (20.8 juveniles hour compared to =1.8 to 2.3 juveniles hour’), it is unlikely that this slight decrease in abundance alone accounts for a change of two orders of magnitude in benthic octopus numbers from 1991 to 1997. Potential factors that may affect juvenile abundance include: the size of the larval pool produced by benthic adults, the availability of food for juveniles in the water column, or sources of juvenile mortality such as predation. Since juvenile abundance in the years after the large benthic population of 1991 was smaller than previous years (Fig. 6), it seems unlikely that adult abundance strongly influences juvenile abundance. The juvenile's food chain is short: O. rubescens eats zooplankton, which grazes on phytoplankton. Such a relationship makes it feasible that fluctuations in the production of phytoplankton affect juvenile abundances by affecting abundances of the juvenile’s prey. Juveniles feed upon mystids, copepods and ostracods (Adams et al., 1995). Although there were no data available on population sizes of these prey items during this study, the positive slopes between juvenile octopus abundances and chlorophyll concentrations suggests that food may indeed be important. The greater correlation found between octopus abundances and chlorophyll concentrations the previous year may be a result of delayed effects of phytoplankton abundances on zooplankton abundances, and consequently delayed effects on juvenile numbers. Factors that could affect prey abundance include natural nutrient cycles in the Monterey Bay, changes in current patterns or other meso-scale oceanographic processes. Because none of the correlations were statistically significant, more data points are needed positively determine the relationship between chlorophyll concentration and juvenile octopus abundance. Sources of predation on juvenile octopuses are not known, but mortality rate among juveniles is probably high. The lower r’ for the correlation of octopus abundances and chlorophyll concentrations from two years prior may be due to a greater chance for sources of mortality, including predation while in the water column, to occur between the recruitment of one cohort and the production of the next generation of juveniles. More 15 research is required to discover what effects predation may have on abundances of juvenile octopuses in the water column. The final factor that may have led to population fluctuations among O. rubescens along the transect area is the increase in predation pressure from harbor seals. With exception of the El Nino years of 1983 and 1993, California harbor seal populations have been growing every year since the Marine Mammal Protection Act was passed in 1972 (Barlow et al., 1995). An annual growth rate between the years of 1982 and 1994 was calculated as 4.1% (Barlow et al., 1995). However, annual growth rates have been estimated at as high as 14.7% (Oxman, 1995 Harbor seals are benthic predators that consume 2.5-5.0% of their body weight per day (Oxman, 1995). Through such feeding habits, harbor seals may affect populations in the Monterey Bay. Oxman (1995) found that octopus, as measured by octopus beaks in scat, accounted for approximately 35% of prey items found in Elkhorn Slough harbor seal scat during three seasons of the year. Additionally, for these three seasons, octopus was the most common prey item in the scat (Oxman, 1995). Seals forage over the entire depth distribution range of O. rubescens: O. rubescens is found to 200 or 250 m, while seals dive to 500 m (Tomo Eguchi, per. com.). Because harbor seals are opportunistic predators, it is unlikely that the decreases in numbers of beaks found per scat, from 5.5 in the early 1990 to 1.5 in the mid to late 1990's, were a result of a change in prey preference. More likely such decreases in numbers of octopus beaks are reflections of shrinking numbers of octopuses available as prey items. It is difficult to extrapolate the Elkhorn Slough seal scat data to the area of the 1991 and 1997 transects. The Elkhorn Slough seals tend to dive in areas further to the south-east of the transect site, diving in and around the Monterey Canyon closer to Elkhorn Slough (Tomo Eguchi, per com.). Additionally, the majority of dives, 11329 out of 16403 were to depths less than 75m (Tomo Eguchi, unpubl. data). In order to document the impact of seal predation on the transect area, more research is required. Along with the changes in octopus populations along the transect from 1991 to 1997, changes in the rest of the benthic community were also evident. Results from the G-test of independence suggest that relative abundances of species present on the transect have changed significantly over this time period (Table 1, Fig. 4). Additionally, Shannon's Diversity Index indicates a significant decrease in diversity from 1991 to 1997. From the research conducted, it is difficult to conclude what these changes may indicate; however, as seen through the investigation of fluctuations in O. rubescens populations, many factors may be working together to create the community wide changes found along the transect. Conclusion O. rubescens numbers on a transect along the 200 m depth contour in Monterey Bay fluctuated significantly from 1991 to 1997. This study found that the large population of O. rubescens seen along the transect in 1991 may have been partially due to a combination of two factors. The first is lower rockfish predation pressure on octopus populations in 1991 as a result of a large rockfish take in 1990. The second is large 1989 and 1990 juvenile octopus abundances in the water column recruiting to the benthic community in 1991. It was harder to establish what factors may have contributed to smaller populations of O. rubescens numbers in 1997 because of incomplete fishery and juvenile octopus data. However, possible factors include greater predation pressure from increased numbers of rockfishes and harbor seals. Although not specifically investigated in this study, whole transect changes such as increases in relative abundances and species diversity were also observed. While this study has begun to investigate factors contributing to changes in a population of O. rubescens at 200 m, further research is required to elucidate the complex factors contributing to changes in deep sea benthic communities in the Monterey Bay. Acknowledgments Special thanks to Chuck Baxter for providing the inspiration for this work, and Jim Watanabe for helping to fit all of the pieces together, as well as for helping with the nuts and bolts of the paper. Thanks to the many people at MBARI including: Jim Barry and Patrick Whaling for help with ROV coordination, Bruce Robison for juvenile octopus data, Francisco Chavez for water chlorophyll data, and especially Nancy Jacobson for all her help with data base searches and video lab research. Additionally, thanks to the many others who provided information including: Bob Leos and Brenda Erwin at the California Department of Fish and Game, Mary Yoklavich and Richard Parish at National Marine Fisheries, Karen Light at the Monterey Bay Aquarium, and Dion Oxman, Tomo Eguchi and Teri Nicholson of Moss Landing Marine Laboratories for harbor seal data. Literature Cited Adams, Peter B. John L. Butler, Charles H. Baxter, Thomas E. Laidig, Katherine A. Sahlin, and W. Waldo Wakefield. 1995. Population estimates of Pacific coast groundfishes from video transects and swept-area trawls. Fishery Bulletin. 93:446-455 (1995) Barlow, Jay, Robert L. Brownell, Jr., Douglas P. DeMaster, Karin A. Fomey Mark S. Lowry, Steven Ösmek, Timothy J. Ragen, Randall R. Reeves, and Robert J. Small. 1995. Harbor seal (Phoca vitulina richardsi): California stock. Technical Memorandum. NOAA fisheries/NMFS Southwest Region. Dorsey, E.M. 1976. Natural history and social behavior of Octopus rubescens Berry. M.S. thesis, University of Washington. Hochberg, F.G., Jr. and W.G. Fields. 1980. Cephalopoda. In: Intertidal invertebrates of California. Morris, R.H., D.P. Abbott, and E.C. Haderlie (eds.). Stanford University Press, Stanford. pp. 429-444. Houk, James L. 1992. Rockfishes: Overview. In: California’s living marine resources and their utilization. Leet, William S., Christopher M. Dewees, and Charles W. Haugen (eds.). California Sea Grant, Davis. pp. 257. Hunt, James C. 1996. The Behavior and ecology of midwater cephalopods from Monterey Bay: submersible and laboratory observations. M.S. thesis, University of California Los Angeles. Magurran, Anne E. 1988. Ecological diversity and its measurement. Princeton University Press, Princeton. pp. 34-39. Oxman, Dion S. 1995. Seasonal abundance, movements, and food habits of harbor seals (Phoca vitulina richardsi) in Elkhorn Slough, California. M.S. thesis, Moss Landing Marine Laboratories. pp. 125. Sokal, Robert R. and R. James Rohlf. 1981. Biometry, 2nd edition. W. H. Freeman and Company, New York. pp. 691-778. Trumble, Stephen J. 1995. Abundance, movements, dive behavior, hood habits, and mother-pup interactions of harbor seals (Phoca vitulina richardisi) near Monterey Bay, California. M.S. thesis, Moss Landing Marine Laboratories. Table 1. Number and Type of Organisms Found Along the Transect in 1991 and 1997 Common Name t ha Latin Name ha 1991 1997 RED OCTOPUS RedOctopus Octopus rubescens 531 ROCKFISHES Stripetail rockfish Sebastes saxicola Small Stripetail Sebastes saxicola Widow rockfish Sebastes entomela splitnose rockfish Sebastes diploproa Unknown spp. SEA CUCUMBER Parastichopsis Seacucumber DOVER SOLE Doversole Microstomus pacificus REX/ENGLISH SOLE Rexsole Errex zachirus Pleuronectes vetulus English sole SLENDER SOLE Slendersole Eopsetta exilis SANDDAB Pacific sanddab Citharichthys stigmaeus Unknown spp. SKATES Raja rhina Longnoseskate California skate Raja inornata Unknown spp. EELPOUT Lycodes cortezianus Bigfin eelpout Unknown spp. SUNFLOWER STAR Rathbunaster californicus Rathbunaster SAND STAR Sand star Luidia foliolata ASSORTED ECHINODERMS Red sea star Mediaster aequalis Feather star Florometra serratissima Gorgonocephalus eucnemis Basket star URCHINS Allocentrotus fragilis INVERTEBRATES I Tannercrab Lopholithodes foraminatus Box crab Chionoecetes bairdi Loligo opalescens Squid Pandalus platyceros Spot prawn INVERTEBRATES II Seaslug Pleruobranchaea californica Ptilosarcus gurneyi Sea pen White plume anenome Metridium giganteum Spongespp. ROUNDFISH Pacific argentine Argentina sialis Clupeidae Herring Sablefish Anoplopoma fimbriam Lingeod Ophiodon elongatus Unknown spp. BOTTOM FISH Spotted ratfish Hydrolagus colliei Poachers Agnidae EEL-LIKE FISH Pacific Hagfish Eptatretus stoutii Spotted cusk-eel Chilara taylori Total Number of Organisms 1253 Total Number of Species Although no individuals from these categories were recorded when area was normalized to 1 ha, individuals were seen on the transect and therefore included in total number of species seen. 861 Figure Legend Figure 1: Study site in Monterey Bay with transect area marked by solid dark line along the 200 m depth contour. Figure 2: Decrease in O. rubescens population along the transect site from 1991 to 1997 with area normalized to one hectare. Vertical bars indicate standard error. Numbers used reflect abundances found along transect sub-samples. Figure 3: Inverse population changes of O. rubescens and rockfishes along the transect from 1991 to 1997. O. rubescens decrease and corresponding rockfish increase. Numbers used reflect abundances found along transect sub-samples. Figure 4: Species population fluctuations along the transect from 1991 to 199 Figure 5: Fishery impact on the rockfish population from 1980 to 1996. Plot of pounds of rockfish taken per year and rockfish abundance per year (Abundance is determined by pounds of rockfish taken per hour of fishing). Figure 6: Fluctuation in abundances of juvenile octopuses in the water column from 1989 to 1995. Figure 7 Correlation between juvenile O. rubescens abundance and chlorophyll concentrations, data from 1989 to 1995 Juvenile Octopus Abundance"= 0.686 (+ 1.500) + 0.104 (+ 0.455) [chlj, r°= 0.01. Correlation is not significant, P-0.83. Correlation between juvenile O. rubescens abundance and Figure 8 chlorophyll concentrations. Octopus abundances are lagged one year behind chlorophyll concentrations. Data collected from 1989 to 1996. "Juvenile Octopus Abundance"= -0.680 (+ 1.061) + 0.448 (+ 0.311) (chl.],r’= 0.34. Correlation is not significant, P-0.22. Figure 9: Correlation between juvenile O. rubescens abundance and chlorophyll concentrations. Octopus abundances are lagged two year behind chlorophyll concentrations. Data collected from 1989 to 1996. "Juvenile Octopus Abundance"=-0.184 (+ 684) + 0.137 (+ 0.208) [chl.],r°= 0.13. Correlation is not significant, P-0.56. Figure 10: Average number of octopus beaks found in 1991 and 1996 harbor seal scat samples. Sample sizes = 547 and 99. 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