Activity Patterns in Pagurus samuelis and Pagurus granosimanus II. Locomotor Activity Eldon E. Ball Jr. Hopkins Marine Station of Stanford University Pacific Grove, California June 1, 1965 C Introduction It was observed that two local species of hermit crabs, Pagurus samuelis and Pagurus granosimanus, were active in much greater numbers at night than during the day. In order to determine whether the crabs move at different speeds at different times of day the following experiments were carried out. Materials and Methods Concentric circles were laid out on a piece of sheet rock at distances of 20, 30, 40, and 50. cm. and it was placed at the bottom of a pond in approximately 10 inches of sea water. Ten Pagurus granosimanus and ten Pagurus samuelis of approximately equal size were then selected. A small weighted cage (plastic strawberry carton) was used to retain the Pagurus in the center of the circles until the start of the experiment. One minute after the investi- gator had left the pool the cage was raised using a string attached to a pole. The time required for five of the ten crabs to pass out of the 50 cm. circle was then recorded. In a small number of cases one of the crabs clung to the cage and was carried out of the circles. When this happened the crab which was carried away was ignored, and the time required O C for five crabs to leave the 50 cm. circle was taken as usual. The trials were run on even hours (e.g. 1400, 1600, etc.) at various times over a period of 6 days with trials every two hours over a 24 hour period on the last day. Two runs were generally taken with each species at each trial. The species were alternated on these runs. The same crabs were used throughout the 6 days of the trials. Night observations were made by using a ruby bulb, although in some cases the pond was illuminated by light from nearby windows. Results The combined data from all runs (fig. 3) seems to show that the crabs move more slowly at night than during the day, but the otherwise fairly regular trend is interrupted at 2200 when the time required for the crabs to get out of the 50 cm. circle falls off rather sharply. The data for the two species considered individually (figures 1 and 2) are much less regular than the combined data, although' in both cases the same trend of slow movement at night and faster movement by day is evident. Discussion Figures 1, 2, and 3 all show essentially the same trend, with the crabs moving slower at night than during the day. The reason for the interruption of this trend C 37 at 2200 is at this time unknown. Temperature probably does not play a major role in determining these activity patterns, since the greatest activity occurs at night (2200) after the water has cooled considerably. Also, the rate of movement begins to speed up in the morning while the temperature is still falling. Intensity of illumination may well prove to be a major factor in determining how fast the crabs move. In order to draw any conclusions about the possible effects of light or physiologisal rhythms on the rate of locomotion in Pagurus a much larger number of trials would be required, preferably under conditions where temperature and illumination could be controlled. Summary Data are presented on the rate of locomotion of Pagurus granosimanus and Pagurus samuelis at different times of the day and night. There appears to be a general tendency for the crabs to slow down at night, but there is an unusual in- crease in the speed with which the crabs move at 2200. More experiments under controlled conditions are needed. 38 Legends for Figures Figure 1. Time (in seconds) required for 5 of 10 Pagurus granosimanus to pass out of a 50 cm. circle at different Hours of day (width of column indicates number of trials Figure 2. Time (in seconds) required for 5 of 10 Pagurus samuelis to pass out of a 50 cm. cirèle at different hours of day (width of column indicates number of trials) Figure 3. The data from figures 1 and 2 are here combined to give the average time (in seconds) required for 5 of 10 Pagurus to pass out of a 50 cm. circle at different hours oi day (width of column indicates number of trials) 820 0600 1200 180 2400 Time 2oon 0600 1200 240 8 Time 800 0600 1800 240 Time