C Haberman p. 2 Although centipedes are primarily terrestrial organisms, some members of the Order Geophilomorpha have been observed in the littoral zone. One species along English shores, Hydro- schendila submarina, tolerates 14 hours of submersion in sea- water (Binyon and Lewis, 1963). The European species Scolde planes maritimus has been observed to feed upon barnacles and littorine snails (Blower, 1957). Recently, specimens of Geophilus becki, also in the Order Geophilomorpha, were found between the alga Endocladia muricata and iceplant borderline, markers of sea and land vegetation respectively, along the Monterey Bay shoreline. Positive identification was made by comparing col¬ lected specimens with animals previously collected along the Monterey Peninsula by Mr. Roy Johnson, and identified by Dr. R.L. Crabill of the Smithsonian Institution. Unidentified species in the Order Lithobiomorpha were also found. The purpose of this study was to determine the distribution of centipedes near the shore, and to define the habitatt of G. becki. MATERIALS AND METHODS Sites were chosen along the shore between Lover's Point and Pt. Pinos, Pacific Grove, California (fig. 1). During pre- liminary surveys, all centipedes found were beneath rocks, in gravel substrate, so the following criteria were used in choosing study sites and transects. (1) All sites had many rocks. (2) All sites had some gravel in the substrate. (3) Transects were not established in areas containing only large, immovable rocks. C Haberman p. 3 Site 1 was most exposed, receiving direct, unblocked wave action at high tide. Site 2 was least exposed, with most wave action blocked by an outcropping rock cliff. At each site, transects were established perpendicular to shore, extending from the land vegetation, across the area re¬ ceiving splash and spray, to the upper midtide region. Tran¬ sects were 1 meter in width, and divided into 2 meter intervals along the surface of the slope. The erect alga occurring highest in the intertidal region (usually Endocladia muricata) was used to mark the boundary between Zones A and B. Within each defined zone of a transect, all movable rocks large enough to be firmly pressed against the substrate and house a centipede were overturned and counted. (Rocks atop other rocks were found not to conceal centipedes, and were therefore not counted.) The area beneath each rock was examined for centi¬ pedes; some centipedes were visible upon overturning the rock, and others were searched for by digging with a trowel to a depth of 10-15 cm into the substrate under the rock. Along some transects, centipedes were found but not col¬ lected. Those centipedes were counted in the field, and not handled unless found in large groups. Large groups were temporarily sequestered by scooping them up in a jar, and the animals counted while taking each individual out and replacing it on the sub- strate where found. Along the remaining transects, all centipedes found were collected. Individuals and small groups were oounted at the collecting site, while large groups were collected and later counted and measured in the laboratory. Escapees were also counted. Haberman p. 4 For each individual or group of centipedes found, con- ditions of moisture, substrate, type of environment (under rock or in drift) and other organisms found with centipedes were re¬ corded. In addition, average values for these parameters were ae¬ estimated for each entire zone, based upon conditions under all rocks overturned. The composition of the dominant elements of the substrate beneath each rock (sand, gravel, and soil) were recorded using the following categories: (1) Bome present, (2) Present in approximately equal proprtions with another type, (3) Present as the dominating type, and (4) Exclusively present. Moisture under rocks was assessed on the following scale: (1) Substrate dry, (2) Substrate dry at surface, moist beneath, (3) Substrate moist from surface downward, (4) Substrate with water droplets visible and/or wet to touch, (5) Rock and substrate submerged Amount of drift algae in each zone was estimated on a scale from 1 to 5, using the following categories: (1) None present, (2) Present, but scattered and thin, (3) Isolated patehbssof drift covering some rocks, (4) Large patches of drift covering up to 50% of zone, (5) 50-100% of zone is covered with drift approximately 1 footdeep, erdeeper. All surveys were conducted in the period May 12- May 26, 1982, at tidal levels in the field ranging from a lower low water (LLW) level of -1.6 feet to the rising tide of a lower high water (LHW) of 4.3 feet. No zones were surveyed while submerged. Haberman p. 5 RESULTS Results of this study are illustrated in Fig. 2-10. The data for Site 2 (Fig 2) show that Zone C has a density of G. becki significantly higher than any other zone. This zone is characterized by a gravel substrate which is moist to wet at the surface; it is just above the region of maximum drift algae, and contains numerous amphipods. This zone is generally covered once a day during higher high water (HHW). No significant differences or trends were observed between two side by side transects when one was surveyed at LLW and the other surveyed close to LHW (Fig. 3). Several organisms were found in the drift. Sites 1, 3,and 4 had much lower densities of G. becki (Fig. 4, 5, and 6). The zones of highest centipede density for Sites 3 and 4 are characterized by approximately the same conditions as those with the most centipedes in Site 2, while the zone for Site 1 occurs at a higher tidal level, isodryer and farther above the drift, and contains no amphipods. Centipedes of the Order Lithobiomorpha were scarce and found only near land vegetation, except at Site 4. Most centipedes found were on substrata which were moist but not wet at the surface (Fig. 7). However, zones B and 66 which containea majoréty of the goneipedes, are submerged at HHW. The majority of centipedes were found in groups. Zones of highest density contained the largest groups (Fig. 1). Groups ranged in size from 2 to 16 members, and smaller indi- viduals had a higher percentage of membership in large groups (Fig. 8). Size distribution of all measured centipedes is shown in Fig. 9. Mean size did not vary between zones (Fig. 10). Haberman p. 6 DISCUSSION The location of the main body of the G. becki population indicates that the species is part of the littoral or drift community. Since some G. becki were found wandering in loose drift, the centipedes may be feeding there. The number of drift wanderers found may be much lower than the number actually there, due to the relative difficulty of finding the animals in the drift as compared to finding them under rocks. The high density of G. becki at Site 2 suggests that both a large number of rocks and presence of alot of drift provide optimum conditions for this species. Only Site 1 had close to the density of drift at Site 2, and it contained many fewer rocks. The centipede population is concentrated in a zone which is submerged once per day. Furthermore, individuals in Zone B were found while the tide was rising to LHW, and this zone is submerged at LHW. At the Pt. Pinos site, two specimens were found together in Zone B ten minutes before the zone was sub- merged. All this evidence suggests that G. becki do not avoid submergence in seawater, and may be regularly submerged. Most centipedes at Site 1, which was most exposed to wave action, occurred at a higher tidal level than was the case for the rest of the sites, suggesting some avoidance to strong wave action or submergence. The Site 1 distribution may also be in¬ fluenced by the low numbers of rocks in all zones. The high percentage of centipedes found in grospessuggests that G. becki is a social animal, and the high percentage of small cen¬ tipedes in large groups indicates a possible "young care' function for the ggroups. However, the occurance of larger and C C Haberman p. 7 more abundant groups in the most populated zones may be due to crowding. The distribution of centipedes within any zone (at least in daylight at low water) is always patchy since the animals hide under stones, and group size may in part reflect the ratio of population density to the number of suitable rocks present. The size distribution curve shows a bimodal trend; however, this is obviously not a statistically significant result. SUMMARY Centipede populations in the splash zone of Monterey Bay were surveyed, and a high density of the Geophilomorph Geophilus becki was found beneath the rocks in a zone just above the attached alga Endocladia muricata and below a tidal level of +5.6 feet. A high percentage of these were found in groups. Few Lithobiomorpha were found in the splash zone. C ACKNOWLEDGEMENTS I would like to thank Dr. Donald P. Abbott for his extensive involvement in this project at all stages, his enthusiasum, and his very useful criticism. I would also like to thank Mr. Roy Johnson for use of identified G. becki specimans, without which the identity of the critter would have remained a mystery, and I would like to thank Chris Patton for the up close and personal view of the animal using electron microscopy. And of course, I must thank Freya Sommer for putting up with me for the entire quarter. C ). 8 Haberman p.9 REFERENCES Binyon, J., Lewis, J.G.E., 1963. Physiological adaptations of two species of centipede to life on the shore. J. Mar. Biol. Assn. U.K. 43: 49-55. Blower, J.G., 1957. Feeding habit of a marine centipede. Nature 180: 560. C Haberman p. 10 Captions Map of Pacific Grove coastline, depicting study site Figure 1. locations. Combined results for 7 transect surveys at site 2, Figure 2. relating physical and biological parameters to centipede distribution. Data from 2 pairs of transects in site 2. Transects Figure 3. 1 and 3 (Tl and T3) were surveyed close to LLW, while transects 2 and 4 (T2 and T4) were surveyed during the rising tide and close to a LHW of approximately 14.5 feet. Figure 4. Combined results for 2 transect surveys at site 1. Figure 5. Results for a single transect survey at site 3. Figure 6. Results for a single transect survey at site 4. Figure 7. Moisture conditions for individual centipedes. Data from all sites and zones åre combined. Figure 8a. Group size frequencies. Data from all sites and zones are combined. C Haberman p. 11 Captions (cont. Figure 8b. Relationship of centipede size to group membership, All measured centipedes were tabulated, and data from all sites and zones were combined. Figure 9. Size distribution of all measured specimens of G. Becki. Figure 10. Size distribution as related to zonation. Data compiled using measured specimens from site 2 transects only. fig S Municipol Golf Course gee det Ar s Place F6. Shg e Mooorch te 89 Haberman 0. 12 3 J 9 Co g S CSS E T Fio 2 15- GROUP IO¬ SIZE 5- MEAN & RANGE 00- PERCEN! IN-GROUPS 50 0 200 0 — 7 160 OI 5 7 20 80- 7 40- —+ DRIF 4 ALGAE 2- M UBSTRATE 0 2 D 2 Z 0 1 MOISTURE 1 .. 3 J L — WATER. EVEL i Lplanaxis Peshxarepsus ORGANISMS Tegule funebralis EZONE A D424 I L. planaxis Pachygrapsus Amphipods ZONE B I 1 1 Amphipod lsopods Ants ZONE CZON Haberman p.13 sopod Ants Bristletails— . 50 6 0 40 G 30 S — O+ 20 10 50 6 0 40 30 8 E O 20 0 10 e ktaa MOISTURE 3. — S WATEF . LEVEE ORGANISMS Haberman 8.14 T2 T1 — 74 S So —- 0 10 tan I. planaxi» Amphipod lsopods Isopods utvlats Ants Ants Pachygrapsus L.planaxis Bristletails Amphipods Pechvorepsus Teavie funebralis ZONE A ZONE B ZONE C ZONE D ZONE E :.. percent In groups 50 0 0 40 14 30 S Q s— — 20 O O 0 10 O drift algae ——.— -— substrate 15 190 400 moisture - — water — —level — organisms 100 50 Amphipods Anthopleura Tegule fupebralis l.planaxis Lplanaxis Lscutulata Amphipods So E0 Ants Hobermanp.16 L J 6 0 0 40 C 309 Q 20 O 0 100 substrate 100 5 8 moisture water 10 level Anthopleure Pachygrapsus Amphipeds Pachygrapsus L planaxis planaxis organism. Hoberman 9.16 N - 10 Amphipods Ants Ants Bristletars Bristletal 8 N Ants Bristletails ( 110 50 40 30 20 10 percent in groups 0 4 9 O s— O 0 drift algae substrate 8. 50 moisture — water leve organisms Tegulo funebrali: Pachygrapsus Pachyarenzus I planaxis L planaxis Lseutulata L. seutuleta ZONE A ZONE B A Eo Amphipods ZONE C 210 Ants Isopods Bristletalis ZONE L Haberman p.11 K Ants Isopods Bristleteils ZONE ( moisture rating 2 3 5 no. of cemipedes 213 93 O Haberman p. 18 F8 Fig 85 Hoberman P.19 30 20 L 10 E 9 8 10 14 12 16 — NO. OF INDIVIDUALS IN GROUP LARGE INDIVIDUALS=6.0-9.5 cm SMALL INDIVIDUALS=1.6-3.5cm 100 00 50 — Na02 E I G. BECKI INDIVIDUALS f 2 2 30 20 — 10 1 10 — Hobermano.20 L 110 10 o 0 — 0 0 — —— 0o 10 o 11010 SIZE CLASS (in cm) FiglO 0 ( 10 O 1 O E 00 0 (0 No c (0 — L N E 0 (0 10 0 O Q10 DODL O N - — Haberman p.2