Laurie S. Hedden ENTR ODUCTION The gastropod Olivella biplicata (Sowerby, 1825) is common in many localities on the Pacific Coast of North America from British Columbia to Baja California, and has been the subject of several investigations. A large population occurs in and around the harbor at Monterey, California (Smith & Gordon, 1948). Wr. David W. Phillips of Hopkins Marine Station, Pacific Grove, California (personal communication) had noted that individuals, at least those in and near the intertidal zone, undergo more or less regular shifts in orientation and position on the beach with changes in tide level, and suggested a quantitative inves- tigation of this behavior. Shifts in position and activity with tide have been noted in other sand-dwelling gastropods, eg. Oliveancillaria brasiliensis (Chemnitz, 1788) (see Gofferje, ricula) auricaularia (Lamarck, 1810) 1950) and Olivan cillaria (Lint: (see Marcus & Ma reus, 1959). The orientation and movement of Olivella biplicata have been examined in one study (Edwards, 1965), but primarily from the point of view of differences between size classes in natural populations. The purpose of the present study was to confirm, and provide a measure of, the tendency of Olivella biplicata to move downward on receding tides and upwards on incoming tides. Laurie S. Hedden MATERIALS AND METHODS All studies were carried out on the shore just east of Fisherman's Wharf, Monterey harbor, California, an area characterized by a very gentle slope and little wave action, ransect lines parallel to the water's edge I set up three within the intertidal region occupied by Olivella biplicata. The transects, separated by 15-foot horizontal intervals on the beach, were labelled Station A (at the tidal height of +1.1 ft), Station B (+0.25 ft) and Station C (-O.25 ft). On separate occasions, both day and night, observations were made at three selected times before, during and after the period of lower low water (LLW). At each sample time, Olivella at each transect area were examined to see whether they were oriented facing upslope or downslope. Animals headed in any direction upward of a line roughly parallel to the wave front were counted as facing ipslope; conversely for downslope. When any station was submerged by more than 6-8 inches of water, animals found on the sand surface provided the most reliable data on true orientation. When the transect area was exposed at each LLW. leaving a firmer substrate, nearly all the animals burrowed below the surface, but the orientation of the burrowed Olivella could be reliably determined. Along with studies of orientation upslope and downslope, at each observation time quantitative measures were made of Olivella population present at each ransect level, to provide laurie S. Hedden a measure of the actual movement of the population up and down the beach. During each ebb tide, low tide, and flow. four 0.25 m' quadrats were selected on each transect line, in areas not previously disturbed by sampling. At each, a sheet metal quadrat frame was inserted into the beach and all animals. both those on the surface and those bur rrowed to a depth of 3 inches, were counted. Animals were returned to the quadrat of capture immediately after counting. Locations of quadrats along the transect lines were changed with each sample time. Night observations were made with the use of a flashlight covered with red cellophane, since preliminary observations had shown the animals respond much less to this than to white light. Animals which were mating, and animals close enough to an occasional Polinices to be exhibiting escape response (Edwards, 1969) were not counted in the study, pe RESOLI The results are su mmarized in Figures 1 and 2. During the ebb tides, there was a significantly greater number of Olivella pointing downslope than upslope at Stations A and B, while at Station C, still covered by more than one foot of water, a larger number of animals were still oriented upslope. At lower low water (LLW) almost all Olivella at each station pointed downslope. The final counts, taken during tidal flow about three hours later, showed a reversal in orientation at the low station (C), while at the upper stations the majority of the animals still pointed downslope. laurie S. Hedden Actual numbers of animals per unit area at the transects (Figure 1), as based on the sample population counts, showed a decrease in the total number of Olivella at Stations A and B, complemented by an increase in the total number at C, as the tide receded. During the rising tide, there was a reversal of this distribution, and the numbers at A and B increased again while those at C declined. The changes observed in distribution are those which one would expect if orientation upslope and downslope is a reflection of movement in these directions. The variability between the four 0.25 m samples taken at any one time on a particular transect was fairly great. for Olivella are rather gre jarious (Edwards, 1965) and show a clustered, non-random dis ribution. While the differences be- tween populations at given transects at different times are not statistically significant, the results obtained are strongly suggestive of population movement up and down the beach. Data in Figure 1 were obtained at night and early in the day. The results shown in Figure 2 indicate that statistically significant changes in orientation with phase of low tide also occurred during daylight hours. Downslope orientation corresponded to the receding tide, and, as before, there was a reversal of direction with the incoming tide, and most observed animals headed upslope. Laurie S. Hedden DISCUSSION There is a clear indication of a change in orientation of Olivella biplicata in the hours before and after lower low water. Although only the differences in numbers oriented upslope vs. downslope are significant, the variations in population at each level on the beach are entirely consistent with these data, suggesting that considerable numbers of animals move up and down the beach with the tide. This is further corroborated by qualitative field observations. Movement is not highly directional; most moving Olivella follow rather tortuous paths. However, they are capable of travelling up to several meters per hour (Stohler, 1969). The stimuli involved in eliciting this behavior have not been determined, though the possibilities are numerous. There has been some speculation as to what the advantages are to Olivella in maintaining intertidal populations. Edwards (1965) has suggested that intra-specific competition and protection from subtidal predators, eg. Polinices and Pisaster, might force larger animals to move higher up on the beach. In addition, a third possible factor could be the high rate of turnover of detritus in this region of the beach, providing a continuously rich food supply for Olivella. By moving with the tide, the intertidal Olivella population can minimize periods of exposure and spend a correspondingly greater time in such activities as feeding and mating, Laurie S. Hedden SUMMARY The intertidal sandy beach gastropod Olivella biplicata exhibits downslope (seaward) directional orientation as the tide ebbs toward lower low water and upslope orientation as the tide rises. Some seaward and landward movement of beach populations also occurs. These orientation changes occur whether low tides come during the day or the night. VMOUTP ACKNOWLEDGEMENT I would like to thank all the faculty, staff and students of Hopkins Marine Station, Pacific Grove, California. Special thanks go to David W. Phillips for his help which led to this study, and to Dr. Donald P. Abbott for his patience and guidance. particularly in the preparation of this manuscript. Laurie S. Hedden TTTEPA LITERATURE CITED Edwards, Dallas Craig 1965. Distribution patterns within natural populations of Olivella biplicata, their underlying behavioral mechanisms and their ecological significance. Ph.D. thesis, University of Chicago. 1969. Predators on Olivella biplicata, including a species-specific predator avoidance response. Veliger 11: 326-333. Gofferje, C. N. 1950. Contribuicao a zoogeografia da malacofauna do litoral do Estado do Parana. Arq. Mus. Paranaense 8: 221-282. Marcus, E. & E. Marcus 1959. Studies on Olividae. Bol. Zool. 22: 99-188. Smith, Allyn G. & Mackenzie Gordon, Jr. 1948. The marine mollusks and brachiopods of Monterey Bay, California, and vicinity. Proc. Calif. Acad. Sci. 26 (8): 147-245. Stohler, Rudolf 1969. Growth study in Olivella biplicata (Sowerby, 1825). Veliger 11: 259-267. Laurie S. Hedden FIGURE CAPTIONS 1. Orientation and movement of Olivella biplicata before, during and after lower low water (LLW) on two successive days at Monterey harbor, California. Numbers of animals heading upslope and downslope, and number/me, at levels A, B and C on the beach are shown. Transverse lines on the bars of the lower graph indicate numbers of animals in four separate 0.25 m2 samples. 2. Orientation of Olivella biplicata before and after lower 4 low water at Monterey harbor, callfornia. All animals seen at each of the three observation periods occurred between the -1.0 ft and the +1.0 ft tidal levels. 00 Obo ooo 2090 ooc0 000 oo 9011 ooto 9010 o0 000 ooo oogr o — — oo — V — — — L 0 8 Np () Fgure I 5 o 100 00 200 Hine f dag (Psr) k k k t- taaa- 0 Fiquse 2