Barbere Best 1 Feeding Activities Tegula funebgelis b Barösta f Best Intredustien The intertidel regions st Chins Feist provide an abum- danee ef merine plente for a dense pepulation of the prinarily herbivorous gastroped Tegala funebralig (1. Adoms, 1894). sith the varisty of plant foods avsilable, I. funebrells sculd be expsetsd to display preferenees. Foeding aetivity of the large snail population (400/*) could certainly effost Leighten the algal erop within the I. fumebralis sone. (in press) has ustablished food preferanses for several gub¬ litterel benthie gresers and his extensive field observatiens have choun the profound effeet of grezing setivities on tas stending algal erop. The pessible fosd seurees of Tagula funebralls fell into three estegoriess rock enerusting sigse, Baeroscepie 383-310 plant forme, end organie detritus (seo Fen, 1957) on th predominantly granite surfacee. 10 testasa Dest 2 Ia edditien to the food onurese, praferenses, and iho annl'e effect en the littaral algal orep, à quenti tative cetinate ef the fecd camomed by the snail unday laboratory ensditiens ras ebtained. Light was investigetel as a jossible fagtaf. lla nA hi 1) Foad owuree determisstien. One to 3 grans ef an dige fer etonde evregud vith an engrusting fora) vore plased in rlestie diches containing 2 andmals (3 to 10 groms cach, vot weight, with chall). The diches, legsted in a grocchauge, vere contimususly supplied with rumming sorwoter at 10 0. Aag ingestien ef the food uas noted aftey 34 Mure. Agifiom reight less ef the alga, fragaaatation, evidenop of radulas resping, of idestifiestien of the feod in the gat og foonl geterial vas used to determins chether er sot ingeotien has ossurred. All enimals and fosts usod voro taken froch fron the field. To piants listed in Table 1 were Ingecdcd zu verving angante and ere therofere osasiderod feod sourede. 4 eimplifisstien ef the erganie carben dstersinatica in (Srick sond, Josl, aud Patsons, T.R., 1960, pp 117-121 was used to seazure Analreis the Maulft the erganie detritus, er leptepel, om tho gramite gurfagss, and therefere the availebility of leptopel as a ford sougen. Sameles vere collested by placing a plestie eylinder uprigja egeinst the grenite zurfaces, aesled at tho base wich plastil. sane medeling elay. Five mi of 1.0 f. Hoh aud 3 - 3 m Sietilled water unchings vore sussessively pedred into us Sestese Best lisg elnder. The as epaed ty ligt r es the rock euffese with a glass rod to simalate ihn raspiag ef Fe fabralis. Cne ni of 70 phosphorie seid vas addes to te sasple and hasted far 2 simtes in e beiling mater bath, fellewed by 10 ni of petasslum dichreuste in sulfurie scie seltties asd 60 mimtes of additienal heating, Atar ng ue to 2 si velume with distilled vater, the sasple was roaa es the Hett celerisster, uupplied with a blus filter. The reswit is given in ug ef earbon, taken fren a standard carve ef glucdee sarbon. The sumplee vore token fron barran rocka, 3te 4 foot abero ILd. Beinz the sans techmigse, the ergenie carben content er a esndstoss sample (sarfacs serapings dova t0 2 u) uns femd. The stene was taken fros the intertidal on the norta side of Pusta Banda naar enponsds, Baja California, ana 1a prebably a food soures fes the leeal Lemia milina (Forbeg 18) peyalatiea. Thie warn water region has an simpge auelusivaly sandstene eubstrate with a fou petches ef broun, filenenteus elgse. Enaminsties ef the fesal naterial from cevral f. malling ehoved apprentntely 004 sandstene ky velune. ne teneining 20f scntsinsd fragneste ef the filemestous eiges, The ergasie content ef foud grenito cusfage cenplos eeraged pa Ge raaging fres 2 t9 67 pa 0/. Te fou n ave 10 O, i ta a 0 e 22 20/2 renge. Sarbara Best 2) Feod preferense determinstien. Preferenee experimenta vere restricted to the meerossopis elgas. Plastie diahee vere again used in the greenbouse. Three sots of algee were effered en alternate days to the sams animals, which had bean collestel ene day before starting the series. The four species of algee in each set were plased, in combinatiens of three, in the dishes with 10 animals (range 1 to 4 grams) or 3 (range 6 te 12 grams). Wet weight differenees of the algae to the nearest O.l gram were taken after 24 hours. Control dishes showed ne appreeiable change in wet weight of any alga (maximum of 0.03 grams). Results are shoun in Cable II. 3) Feeding rates. Maeroexstie integrifolia blades were selested for the feeding rate detormination because of the sccessibility of its large blade area and its apparent appeal to T. funebralis. Andmals collected in the field were fod M. integrifolia for 36 hours before beginning. The wet weight of the alge conzumed vas weasured 6 times at intervals of Ste 23 hours for a tetal of 82 hours. Two size ranges of the snail vere again selested (3 to 5 and 7 to 9 grams) vith four enimals in each dieh. Rezults are eenverted to graus of the algs sonsumed per dey per gram wet weight (with shell) ef 1. funchralis (Table III). Ihe contrel algae chowed a naximm wet weight change of 6.02 graus. 4) Effeet ef grazing en the litteral algal crop. Fer this Darbarn Best 9 shert peried etudy (17 daye), vire nech eagee, 30 x 20 n 30 a, were fastened over the subatrate vith the basse fluah to the rocks. Four cages vare placad adjasantly (2 in each of 2 erese). Togala funebralig vae eneluded from one oege and 20 to 200 anisale vere enelosed in the adjadant sage of esoh Arse. At the and of the study, tho only apprecieble changes tut occurred wares a) neticable grazing on Rederlogsun afing, Gelidiun 2p., and iridsna flessideg b) the appearende of Mvs s2. in one of the seges withest anisele (the alge vas feund novhere else vithin a radius of over 3 matore) Diequssion Though Regule funebrelig vill ingest almost any opanen elge, it displeys dafinits preforengse among the Eseroscopie forma, i.e. Maerooystie integrifolie, Hersogystig Lustkane, Rodoglessug affing, and Cigartine sanaliggleta. in saan esse, the preferred elga is a eflechy" form. It ia non- salearious, noo-filenentous, and has a tender thallus sontrastal to the lasthery testure of Gigartima Aghardii and Pelyetia fastigistg. The proferenses are consietent in the two eire groupe of T. funabgalle. sinee the highly preferred gient brovn sigae are normaliy aveileble to the snail populstion only as drift paterial, the food sourecs of the enimals inelude plante groving outside the f. fumebrelig sone. Thie is supported by a fleld altustien Berbara Best et a site of organie detritus sampling. The T. funebralie population at this leestion dvells en extremely barren rocke and is apparently nourished by the considerable quantities of drift seavseds thet secumulste. The enerusting algse, partieularly the abundant green algs and Balfeis pacifies, are undoubtedly inportant in the diet of the animal. As Figure 1 illustrates, the width of tho resping pattern on a glass plete thinly costed with distoms greatly exeeeds that found en a thick Hacroeystis intsgrifelie blade, sheving en effisient nechanies for oovering a lerge aurfase area then necsssary: Thie may be inportant in keeping larger algee free of diatos grovth. Organie detritus on granite surfacee is probably enly an sccidental food sourse at best. The detritus does contain 90 2- digestible compounds, bowever (see Nigrelli, 1963). Tegala gellina undeubtedly utilizes the orgenie contsnt of its candotone substrate in s menner sisilar to Littorina planazig (Thilippi 1847) and L. seutulata (Oould 1849) as shown by North, 1934. In esrlier work, North (umpublished data) meszured the erceite setivitiee of T. funebralis en sandstone. Re colleeted 1.109) ams of eroded (ingested) sandstone from the fecal seterial of 11 sneils (total weight 58.4 grams) in 24 hours. Orgenie detritus, as found in sandstone, may therefore be inoleded se a foed couree of T. funebralis as vell as T. gellins, though sendstone is not found in the Chins Point area. Barbarn Best7 Table III eleerly shavs that the larger snimals heve a levee fesding rate then the eæaller snails. The overall differenee ef 17% in feeding rate found between sunlicht and constant darknecs is toe small to support the possibility of a photo- kinetie reeponze in feeding sctivity. The mean feeding rute in light (0.012 gne/ge of animal/day) probably represents the meximum found in the field, einee the feed was evsileble in exesse and shown to be highly preferred. The cenditien of the algse in the esgee at the end of the 17 dey perlod (in experiment 4) verifies the preferenecs determined in the laboratory. Lach of the species of algae effected could be called a "fleshy" form. A definite relationship between the Tegula funsbralis populatien and the marine plants in ite envirenment is certeinly indiseted. Sery 1) Teguls funebralis vill ingest nearly any alge groving in er out of the litteral zone. Both seeroscopie and anerusting forms constitute importent food sources; the grenite surfeces contsin s negligible smount of erganic detritus end contribute littie to the diet of the anisel. Karlier wosk on I. fumebralig and observations of I. gallina show that the animals will ingest ergenie detritus when it is aveileble. 2) The snail profers a 'fleshy9 alga azong mecroscopie foras (e.g. Macrocystiz int grifolie, Jereoeystis Luetkenne, and Sigertina sapalioolete). O Barters Best 2) Feeding rates per gren ars gretter in the smeller anisale atkot and ere apparently not affected by sunlight. er auggestzthe importanee of the feeding 4) A short field st setivitios of T. funabralig on the local slgal erops, C Bebang de Ladle 1 Flamt foots ingested by Tegula fusebgalis Chsetemerpha ep. Pelvetia fastigista Cledephora ep. Peystennelia paeifies" Oerellina ep. Fhylleepadiz Seeuler! Mater) Prienitie lanosolata Egregis Menziesii Ralfeia pacifiea Endocladia muriesta Rbedeglessun affine Castroelonium Goulteri Ulra ep. Celidius sp. Unidentified untsellujer green) alga grewing on reeks Cigertins /Oghardii 1 Gigertine esneliculate lEnerusting alge Irideee fleceidm Atale far tlsn Lithothennion sp. kaus ale aer lu Hecroeystie integrifolis Rereocystis Luetkeana Siutt (1744 Table 11 Feod preferences ef Tegula funetgalis Experizent Aneber ef andmale0D 1 3 k l. 10 0.3 0. 2 „ 0.0 2.0 0.1 n 0,0 2.3 0.1 = 0.1 1 0.3 2 0.1 0.0 » 0,1 0,1 0.1 „ 0,0 0,0 6.0 10 0.3 0.2 0.2 u 2.1 0.0 0.0 2 2.3 0.2 0,0 a 29 3 0.3 0. 1 0.8 | 2. 1 a 0.0 0.0 | 2.6 | 2 0.0 0.1 Ftal ceten 1.3 0.7 0.4 C.g 6,2 0.2 0,1 0.1 7,6 0.3 0.0 0.2 Hamber of anizals 3 0.2 0.1 2 0.0 | 2.2 0.1 | 2 0.0 3.0 | 0. 1 1 6.0 3 0.7 2 0.1 0.1 0.0 0.2 0.0 0.1 0.1 0.0 9 0.3 0. 2 0.1 „ 2.7 0.1 0.0 2 2.7 0.0 0.0 1 3 1 0.3 0.1 0.0 | 2. 2 2 0.0 0.0 | 2.6 | 2 0.0 0.0 Tetal enten 1.4 0.6 0.3 0.1 7.1 0.2 0,2 0.0 8.3 0.2 0.1 6.9 8 Pelvetie fastigiete A Cigartina eenaliculata n Sigartina agtardii B Rhodegloszum affind 1 Herescyetis lastkeam 0 Endoeladis wuricsta 3 Gladepbera 29. D Iridace flescidum K Flyllospediz Seouleri I Haereeystis integrifelia L Prienitis 2p. P Orallina s. olant ohilld tiom 8pt Embers represent trase of the plant consumed afteg 24 houge Barben Et Bler . Table 111 Feeding retes of Ieguls funehrelis Sunlight heurs in tezt perieis eseumt conzumed animal weight total rango 8.3 25.3 12.5 12,6 12,3 11,3 total gus day E 4221 7-9 6.18 0.14 0.35 0.16 0.18 0.12 1.13 0.33 0,010 34.9 7-9 0.06 0.2 0.64 0.13 0.12 0,05 1.30 0.38 0.611 15.5 0.06 0.Z 0.3 0.00 0.11 0.10 0.90 0.2 0.017 14,9 35 0.17 0.2 0.10 0.12 0.12 0,07 0.78 0.23 0.013 Ceastant Derkness 32,1 7-9 0,03 0.26 0.4 0,02 0,01 0.2 1.07 6. 0.010 35.7 7-9 0.14 0.00 0.00 0.2 0.01 0,12 0.47 0.14 0.004 13.1 35 0.00 0.19 0.2 o.11 0.07 0.07 0.69 o.20 0.013 16.3 33 0.0 0.38 0.02 0.06 0.28 0.13 0.92 0.27 0.017 Numbers represent the grass ef Maaroerstiz intsgrifelis conzuned in the given time period. Horisontal rows give data fog å saimals, with total weights and weicht ranges. (in vet weicht of animals, with chell) 0 B1 Figure 1 Legend a, Rasping pattern on glass plate, thinly coated with diatomsX tattern on Macrocystis int grifolia blade X B .. /