ISTRIBUTION OF MARINE ALGAE THE VICINITY OE A SEWAGE OUTFALL AT CARMEL, CALIFORNIA Introduction For years, the standard method for an assay of pollution has been a test for the presence of coliform bacteria, under the assumption that these indicate the presence of other, more harmful bacteria. While this test may be useful from a public health viewpoint, it fails to indicate the presence of sewage itself, which these studies show, may contain more materials harmful to the environment than coliform bacteria themselves. An ideal test or "indicator" of pollution should meet two criteria:be obtainable at all types of outfall areas and be repeatable. The coliform test conforms to the first, but is variable on repetition. This paper will suggest a new type of indicator, that of varying algal distribution around an intertidal outfall area, as a helpful measure of the extent of total pollution. It must be emphasized that this is a correlational study, and that to be entirely accurate, further field and laboratory tests would have to be run on the algae in question to verify that it is the effluent that is re sponsible for the distributional differences. Douglas Grey Comparitively little has been done concerning the distribution of marine algae, especially around an outfall Doty (1949) summarized the tidal effects on algal vertical distribution and did some work in the Carmel area, before the present outfall was built. His work, however, includes only conspicuous genera and covered such a wide range of coastline that this could not be used as a control. Ricketts and Calvin (1939) give transects in much more detail, but do not cover polluted areas that surround an outfall. The Carmel outfall, Carmel, California was chosen as the sample study area. It is situated on a rocky point just south of the Carmel River mouth and about one mile north of Point Lobos State Park. There are rocky inter- tidal areas (see fig. 1) within 1000 yards to the north and most of the way to Point Lobos to the south. There was an ideal gradient of natural population densities of marine algae. Mapping and Current Studies In thepreliminary tests, I was one of a group of five students at Hopkins Marine Station studying the Carmel putfall area. The initial research, that of mapping the area around the outfall and studies of ocean currents in the Carmel Bay, was a necessary prerequisite to all of our individual projects. Using a measured line and compass the immediate outfall area was mapped (fig.2) showing in detail, rocks, tidepools, etc., 150 feet to either side Douglas Grey of the outfall pipe. A map (fig. 1) of a larger area was obtained from the California State Department of Parks and Recreation which showed the area from Mission Point (approx. 2000 feet north of the outfall) to Point Lobos (approx, one mile south of the outfall). Following this mapping, an extensive current study was done of Carmel Bay south of the Carmel River. Both fluor¬ escein dye packets and 12 plastic bottles filled with fresh water were used for each run. The dye packets were helpful around the immediate area and the bottles were more useful for the offshore currents. The dye packets were thrown into the water and followed up to 1/2 hour, while the bottles were dropped in a pre-determined pattern and sighted from several reference points at 15 to 30 minute intervals. The bottles were recovered after two to three days, if possible. Figures 3A-E give the results of the current studies. Around the outfall, there is a general flow towards the beach, which then turns north, circles back toward the ocean, and then heads directly south. The offshore currents go directly south and gyre offshore from Monastery Beach. ur current studies showed that the effluent missed the rocks just south of the pipe, going far enough offshore, but being carried back into shore by the gyre. This was verified by the results of our chemical tests, this station giving consistently lower concentrations of the effluent constituents than stations further south, The recovery percentages were 33% for the first current study and 58% for the second. 278 Douglas Grey Chemical Analyses Chemical analyses of the effluent at selected stations around the outfall area (fig. 4) were then made for later comparison to the algal distributional studies. Tests were made for eight parameters. Micro gram-atom concentrations per liter were calculated for phosphates, nitrites, and nitrates by standard methods (Strickland and Parsons) as well as milliliters of dissolved oxygen per liter using Winkler titration (Strickland and Parsons). Salinity was measured with a refractometer, and hydrogen sulfide and chlorine were determined by iodine generation and thiosulfate titration with a starch endpoint. Temperature was meas ured with a standard centigrade thermometer. The results ofthe chemical tests are summarized in figures 5-8. The first station "A" is at the sewage plant itself, and, after that, the stations correspond to the geography, going from Mission Point at the north, through the outfall area, and down to Point Lobos to the south. The concentrations obtained through the tests verified the direction of currents as studied. The values of nitrites, phosphates, hydrogen sulfide, and chlorine were all at their highest values at the plant, and show gradual dilutions going south, with apparently no effluent going to the north. Douglas Grey There were differences in the rates of dilution of the constituents, phosphate and salinity diluting the fastest, while nitrite and chlorine residues were detectable in measurable quantities at North Point Lobos. The nitrite given off by the outfall is oxidized to nitrate, and these describe reciprocal curves on the graphs. As previously stated, the low values at station E, just south of the outfall, are explained by the cur- rent pattern, showing the effluent missing the station. Algal Distribution Marine algal distribution was studied both quali tatively and quantitatively. The qualitative aspect was studied through the use of transects, imaginary Lines running from the highest growth of marine algae to mean lower low water. All algae within 1/2 ft. on either side of these lines were noted on a cross-sectional map of the rocky intertidal area being studied. If an alga looked unhealthy, this too was noted. Transects (fig.9) were taken both north and south of the outfall area, ranging from Mission Point to Point Lobos. The quantitative distributions were obtained using wooden squares 1/2 meter on a side, the area studied each time being 1/4 meter2. All marine algae within the square were noted and again, if there was a definite structural injury to the plant, this was also noted. Douglas Grey The selection of these distributional squares was not random. Areas of five "characteristic types" (described below) were chosen at each of eight transect stations (excluding 3 and 5): 3-4 feet above mean lower low water horizontal inshore direct sun 2-3 feet above mean lower low water vertical not facing waves 2-3 feet above mean lower low water II. vertical facing waves inshore 3-4 feet above mean lower low water horizontal standing water, direct sun near wave action 4-5 feet above mean lower low water horizontal rocky, few plants direct sun, splashed at high tide Wost algae were identified in the field, but some were taken into the lab and identified there. The data from the transects are contained in fig.10A-J The cross-sectional map appears at the top, the species found are listed on the left margin, and their approxi mate area of occurrence indicated with either half or a full bar, relative to subjective population count. Figure 11 shows the distribution of algae in these transects and is a summary. A bar is placed in the appro priate transect column showing occurrence. The length of the bar is proportional to the population present in the 275 Douglas Grey transect. The species are arranged with the species occurring furthest away from the outfall listed first and those occurring mainly at the outfall listed last The empty bars indicate noticeable physical damage to the algae, e.g., little side-branching, dryness, or stunted growth. The transects provide useful information concerning the presence and qualitative abundance of marine algae at various transect locations. These have an advantage over the distributional squares because they cover a much larger area and provide a more general idea of algal distribution around an outfall area relative to tidal height and wave exposure. Their primary value is contained in their summary The "V-shape" of the summary table in figure 11 in dicates varying tolerance of marine algae to the effluent. Codium fragile, Gastroclonium coulteri, and Postelsia palmaeformis all appear in transects at a great distance from the outfall. Hildenbrandia occidentalis, on the other hand, appears in every transect, being extremely dense at the outfall area. The algae listed between these exhibit a gradient of resistance between algae like Codium and Hildenbrandia, suggesting the hypothesis that marine algae do have varying resistances to effluent constituents, ranging from the type of alga affected at ven small concentrations of effluent (which may be cal Douglas Grey culated from the results of the chemical tests) to others which may withstand that same concentration of effluent. Tables 12 A-E show the results of the distribu tional squares. Each species is followed through one of the five types of locations, plotting the area covered versus the distance from the outfall. The para meter of area covered is assumed to be equivalent to the suitability of the environment to growth of the alga. These should quantify what was hypothesized by figure 11 and should give evidence supporting the use of certain types of algae as indicators. These tables contain only algae whose gradients seem significant. The entire list of species appears in the appendix. Concerning the individual species, Gigartina canal iculata, appears to be the most sensitive to any concen¬ tration of effluent, since it occurs only on the seaward side of Point Lobos. Leathesia difformis and Postelsia palmaeformis exhibits slightly higher tolerance, their first appearance still 4000 feet south of the outfall. Both Gigartina canaliculata and Leathesia difformis are common throughout the Monterey area. Both the articulated and crustose corallines show a tremendous gradient (fig.12 B). Mission Point, one of the control areas, shows crustose coralline covering an area of over 2000 cm. This value drops to zero behind the outfall and again rises to over 1600 cm2 at South Point Lobos, the seaward transect. Douglas Grey Gelidium spp.in three of the five population distributions showed a similar gradient. Hildenbrandia occidentalis shows a reverse gradient (fig. 12 B) going from a control value below 100 cm, up to 2250 cm in an area near the butfall, and back doun to 80 cm? in the control area on the opposite of the pipe. Porphyra lanceolata shows this same gradient, but not with the same dramatic figures. The distributional squares show a correlation between distance from the outfall and total number of species in the square. (fig. 13) There exists a direct relationship, i.e., few species close to the outfall and more species as the distance increases. The exception to this, distri¬ bution type V in figure 12 E could be explained by the higher intertidal organisms having less direct exposure to the effluent. Organisms living closer to the lower low water level are more constantly washed by effluent. One correlation for further investigation is that between the chemical concentrations found at various locations and the algal distribution in those same areas. If one could begin his tests on the effects of algal viability of the effluent at the next higher concentrations than was detected at the area where an alga first occurred in the distributions, it might be possible to ascertain exactly what constituent or con- stituents are limiting its environment and at what concentrations. Douglas Gre As for the use of any of these types of algae as indicators, much more research would have to be done, at different outfalls, under different conditions, and over longer periods of time. The algal distributions presented here do exhibit gradients which might imply their use as indicators. The corallines, Endocladia muricata, Gelidium spp., Iridaea splendens, Rhodoglossum affine, Gigartina papillata, Gigartina canaliculata, Egregia menziesii, Hildenbrandia occidentalis, and Porphyra lanceolata all show signs of being possible candidates for indicators of damage, but this study was of only one outfall. To formulate conclusions, more work must be done. Douglas Grey Conclusion Both the current studies and the chemical studies verified that the effluent is traveling south, along the coast. There are measureable amounts of some com¬ ponents of effluent up to 4000 feet to the south of the outfall. There is a difference in the rates of dilution between constituents, this unexplained. The algal distributions show effects up to that same 4000 feet to the south, with some algae appearing only utside this distance (Gigartina canaliculata). The algae show varying tolerances to the effluent, appearing at different distances from the outfall, corresponding to different concentrations of effluent. Indicator algae must be verified at different locations, but this will be a start. Concerning the Carmel outfall, this dis- tributional study with additional studies will provide a baseline measure of change through time. Douglas Grey Summary Both transects and distributional squares may be used to ascertain algal distribution. Marine algal distribution around an outfall area shows 2. distinct variations, dependent on the direction of effluent flow. There exists a varying resistance to the effluent in different species, and within the same species. The gradients shown by species of algae may lend them¬ selves to use as pollution indicators, but more work must be done to minimize the variables of this study. Douglas Grey Acknowledgements would like to thank Mr. Rich at Point Lobos State Park for access to that area and Nr. Mehlert of the Cali- fornia State Department of Parks and Recreation for the Carmel Bay map. My special thanks go to Dr. Isabella A. Abbott for help with identifications and aid in the writing of this paper, but mainly for her encouragement during the entire duration of my research. This study was ipported by Undergraduate Research Program of the National Science Foundation, grant GY-7288. 2 Literature Oited Maxwell (1946) Critical Tide Factors that are Doty, correlated with the Vertical Distribution of Marine Algae and other Organisms along the Pacific Coast, Ecology, Vol 27 No. 4, 315-328. Ricketts, Edward F. and Jack Calvin (1939), Between Pacific Tides, Stanford Univ. Press. Strickland, J.D.H. and T.R. Parsons (1965), A Manual of Sea Water Analysis, Fisheries Research Board of Canada. igur Southern half of the Carmel Bay XX CARMEL BAY S S .. JPOINT LOBOS MISSION POINT OUTFALL Tr MONASTERY BEACH Scale 1:10,000 8 gure ? The immediate outfall area — — — I ) O O N outian 1 O . — O 2 — p Figure 3 A himedite ua ara The currents around the — ..-.-------—— -- S --—.-----—— — --.- T — — S L ... 290 A 45 Figure 3 Mappings of Current Study One, done on May Vrg 272 Carmel Bay CURRENT STUDY I May I1,1970 0930-1600 Mission Point e â e Pt. Lobos O REF.PT.! Outfall OREE. PT2 ku Monastery Beach 1.10000 Scale Rigure Results of the second Current Study, done on May 4 Mission Point Carmel Bay Mn CURRENT STUDY II May 25,1970- -IIOO-1300 -.. - - ----- 4 -4 7 t w 4 er Pt. Lobos Scale Outfall 0 Monastery Beach 1:10000 27 N 4 igure Readings for both of the current studies Time 1032 1039 1102 1122 1132 1142 1232 1252 Time 1115 1130 1145 1215 5 12 Ref. Pt. Z 2 — L 2 Ref. Pt. 3 CURRENT STUDY I Bottle No. 2 222° 233 215 207 200 205 212 185 197 206 215 218 210 202 222 185 227 216 230 233 222 214 210 206 214 213° 205 212° 200 208 218 209 206° 215 215 88 ° 205 212° 215 205 208 210 216 178 198 200 185 193 195 202 187 187 195 182 187 190 188 189 CURRENT STUDY II Bottle No. 4 2 330 925 345 310 12 35 345 34 342 0 350 350 35/ 358 350 5 353 8° O° 355 0 10 3° 50 34 22 15 /2 20 322 337 345 35. 30 9 236 224 214 215 210 202 195 195 320 335 339 354 12 10 244 254 260 238 229°230 248 240 24 224 248 238 235 228 218 224 230 210 199 200 183° 20 10 12 315 3/6 318 334 330 335 337 335 336 350° 350 353 3 358 358 Figure 3 Recovery information for both of the current studies Study II Bottle No. 12 10 11 12 Location Monastery Beach N. Pt. Lobos Monastery Beach prox. 50 yds. off north end of Monastery Beach Date 5/21/70 5/25/70 5/22/70 5/27/70 5/27/70 11 11 295 igure Stations for the chemical tests 2 . x Carmel Bay LOCATION of SAMPLES for CHEMICAL TESTS —I S Pt Lobos )3— 5—A. Sewage Plant B. Mission Pt. 3 D. Outfall G. H. Monastery Beach -I. N. Pt. Lobos Scale 1: 10,000 30 Results of the chemical tests 5 252 35 30 25 20 510 Salinity ao A — A Station H Station Chlorine — S ào 3 ABCDEE Hyd rogen Sulfide GH 0 — A B Phosphate DEFG H 61 700 600 500 400 300 Station 303 Z ao A Nitrate Nitrite H Z ao 25 20 15 10 A D E Station E Station H —o 304 d 2 25 15 12 6 v¬ Dissolved Location Location L Oxygen 6 H Temperature 10 35 igui Locations of transects O6 3 —10 Mission Point Carmel Bay LOCATION of TRANSECTS Point Lobos ... 34 Outfall Monastery Beach Scale 1:10000 30 Figures 10 Results of transects taken in outfall area. The cross section appears at the top. The bars are proportional to subjective population counts. 30 . 75 25 5 Species Agardhiella coulter Alaria marginata Cladophora spp. Coralline-articulated Coralline-crustose Cystoseira osmundacea Egregia menziesii Endocladia muricata IEnteromorpha spp. Fucus distichus Gatgore Gelidium spp. Gigartinaagardhii Gigartina papillata Hildenbrandia occidentalis Iridaea spp. Laminariasinclairii Pelvetiafastigiata Prionitis lanceolata Phyllospadix Ralfsia pacifica Rhodoglossum spp. Ulva lobata 34 DisTAce on HHW (g E 760 1 70 30 D 5 1o 25 Species Agardhiella coulteri Coralline-articulated Coralline-crustose Egregia menziesii Endocladia muricata Enteromorpha spp. Fucus distichus Gelidium coulteri Gigartina agardhii Gigartina papillata Hildenbrandia occidentalis ridaea spp. Laminaria sinclairii Peyssoneliappadffica Phyllospadix Porphyra lanceolata Prionitis lanceolata Rhodoglossum affine Ulva lobata a 75 90 3 DISTANCE FRAN HHNE7) E 50 5 4 3/0 71oo 5 X 00 D 2 40 Darxce rea dW 1er Species Alaria marginata Corallina-articulated Corallina-crustose Cystoseira osmundacea Egregiamenziesii Fucus distichus Gatgore Gigartina papillata Grateloupia setchelli Hildenbrandia occidentalis Iridaeaspp Laminarialsinclairii Phyllospadix Porphyra lanceolata Prionitis lanceolata Ralfsiatpacifica Rhodoglossum affine Ulva lobata 60 70 3/ 9. 952 34 -2 2 5 Species Cladophora trichotoma Coralline-articulated Coralline-crustose Egregia menziesii Endocladia muricata Fucus distichus Gatgore! Gelidium coulteri Gigartina agardhii Gigartina papillata Hildenbrandia occidentalis Iridaeaspp. Pelvetiopsis limitata Porphyralanceolata Prionitis andersonii Rhodoglossum affine Ulvatlobata Heterochordaria abietina Callithamnion pikeanum . DISTANCE FeO AAW (er) 6 X o1 45 20 . Species Coralline-articulated Coralline-crustose Egregia menziesii Endocladiamuricata Enteromorpha spp. Fucus distichus "Gatgore! Gigartina agardhi Gigartina papillata Gelidium coulteri Grateloupia setchelli Hildenbrandia occidentalis Iridaeaspp. Laminaria sinclairii Porphyratlanceolata Prionitis lanceolata Heterochordaria abietina Ralfsiapacifica Rhodoglossum affine Ulva lobata 6o 7/40 116 S 2 7 129 Disraxce Aoe HHW (er) 3/3 9. 952 5 10 20 30 Species Cladophora trichotoma Coralline-articulated Coralline-crustose Egregialmenziesii Endocladia muricata Fucus distichus atgoe Gigartina agardhii Gigartina papillata Hilldenbrandia occidentalis Hridaeaspp. Laminaria sinclairii Porphyra lanceolata Prionitis lanceolata Phyllospadix HUlva lobata 100 3/ 15 X 2 /0 20 Species Cladophora trichotoma Coralline-articulated Coralline-crustose Endocladia muricata Fucus distichus Gatgore Gigartina corymbifera Gigartina papillata Hildenbrandia occidentalis Iridaea spp Pelvetia fastigiata HPhyllospadix Prionitistlanceolata Rhodoglossumaffine +Gigartina agardhii 30 40 30 DISTANSE FRON HHW 70 3/ 9 5 232 2 00 7. S0 -2 30 10 20 Species Coralline-articulated Coralline-crustose Cystseira osmundacea Dictyneurum californicum Egregia menziesii Endocladia muricata Fucus distichus Gatgore Gelidium coulteri Gigartina agardhii Gigartina corymbifera Gigartina papilllata Hildenbrandia occidentalis Hridaeaspp FLaminaria sinclairii Pelvetia fastigiata Porphyra lanceolata Prionitis lanceolata Phyllospadix Rhodoglossum affine E DiSTANE Eon W 70 80 3/6 95 S4. §2 2 S 10 20 30 50 Digevce ee HHNe Species Agardhiella coulteri Alaria marginata Coralline-articulated Coralline-crustose Callithamnion pikeanum Cladophora trichotoma Cystoseira osmundacea Egregia menziesii Endocladiamuricata Gigartina agardhii Gigartina californica Gigartina canaliculata HGigartina corymbifera Gigartina papillata Gatgore" Gelidiumoulter Heterochordaria abietina Hildenbrandia occidentalis Iridaeaspp. HLaminariatsinclairii Leathesia difformis Macrocystistintegrifolia icrocladia coulteri Pelvetialfastigiata Phyllospadix Porphyralanceolata Postelsia palmaeformis Prionitis lanceolata Rhodoglossumaffine Ulva lobata Fucus distichus 68 70 2 8/7 952 X 00 8 1/ Species Agardhiella coulteri Alaria marginata Callithamnion pikeanum Cladophora trichotoma Codium fragile Coralline-articulated Coralline-crustose Cystoseira osmundacea Desmarestia californicum Egregiatmenziesii Endocladia muricata Enteromorpha spp. Fucus distichus Gastroouu Gelidiumspp. Gigartina agardhii Gigartina californica Gigartina canaliculata Gigartina corymbifera Gigartina papillata Hildenbrandia occidentalis Iridaea spp. Laminaria sinclairii Macrocystis integrifolia Microcladia spp. Pelvetia fastigiata Peyssonelia pacifica Phyllospadix Porphyra lanceolata Postelsia palmaeformis Prionitis lanceolata Rhodoglossum affine Ulva lobata 30 40 50 DISTANSE FRO KHW 70 190 J Summary of the transect information Each bar is proportional to population found in the transect. Empty bars indicate visible physical damage. 952 X SPECIES Codium fragile Desmarestia herbacea Gastroclonium coulteri Macrocystis integrifolia Postelsia palmaeformis Agardhiella coulteri Callithamnion pikeanum Dictyoneurum californicum Gigartina talifornica Peyssonelia pacifica Alaria marginata Cladophora trichotoma Cystoseira osmundacea Gigartina canaliculata Gigartina corymbifera Leathesia difformis Microcladia spp. Pelvetia fastigiata Phyllospadix Coralline-articulated Coralline-crustose Endocladiamuricata Enteromorpha Spp. Egregia menziesii Fucus distichus Gatgore Gelidium spp. Gigartina agardhii Gigartina papillata Hildenbrandia occidentalis Iridaea spp. Laminaria sinclairii Porphyralanceolata Prionitis lanceolata Heterochordaria abietina Rhodoglossumaffine Ulva lobata Grateloupia setchelli Ralfsia pacifica Laurencia spectabili Total of Species 27. 22 Transect Numben 5 20 23 24 21 9 3 10 Mr 32 Z 29 31 2 37 9. Tables 12 A-E Tables of results of Distributional Squares - note gradients through the outfall 32 Population Distribution Description of the area: 4-5 feet above lower low water horizontal inshore direct sun Area Covered (cm /m./4) Location Species Leathesia difformis Heterochordaria abietina Egregia menziesii Callithamnion pikeanum Corallines-crustose Gigartina canaliculata Iridaea Gelidium Endocladia muricata 300 170 25 Corallines-articulated 25 20 Gigartina papillata 25 50 375 Ulva lobata 15 475 Cladophora 300 1250 1000 Porphyra lanceolata 40 20 60 500 375 360 1250 320 750 625 50 85 320 280 120 10 450 500 250 200 180 225 Population Distribution I Description of the area: Vertical 2-3 feet above lower low water offshore not facing waves Area Covered (cm /m /4) Location Species Gigartina canaliculata Pelvetia fastigiata Leathesia difformis Agardhiella coulteri Iridaea flaccida Rhodoglossum spp. 190 Gigartina agardhii Egregia menziesii 2250 60 200 100 Gelidium spp. 150 250 Endocladia muricata 175 Callithamnion pikeanum 150 20 150 Gigartina papillata 90 6600 50 70 Iridaea splendens 750 2250 40 700 Corallines-crustose 750 75 140 100 30 Corallines-articulated 100 40 Porphyra lanceolata 15 1250 2250 125 Hildenbrandia occidentalis 35 150 200 120 Ulva lobata 250 10 625 50 625 300 25 50 900 600 450 625 830 390 3000 20 100 155 20 370 150 1500 1625 750 1250 500 40 80 250 75 Population Distribution Description of the area: vertical 2-3 feet above lower low water inshore facing waves Area Covered (cm /m /4) Location Species 240 digartina canaliculata 750 Pelvetia fastigiata 20 Leathesia difformis 130 Rhodoglossum spp. 100 Agardhiella coulteri 300 3000 30( Egregia menziesii 100 100 200 27 Gigartina papillata 320 30 Gelidium spp. 100 700 1000 200 Endocladia muricata 420 750 1000 400 120 150 Iridaea spp. 70 1000 50 700 320 110 70 Corallines-articulated 1500 1125 40 100 100 60 200 Corallines-crustose 275 625 30 90 300 256 1750 70 Hildenbrandia occidentalis Population Distribution IV Description of the area: 3-4 feet above lower low water heavily populated standing water direct sun near to wave action Area Covered (cm /m /4) Location Species Gigartina canaliculata Postelsia palmaeformis Agardhiella coulteri Microcladia spp. Rhodoglossum spp. 160 Prionitis lanceolata Gigartina agardhii Iridaea spp. 100 50 240 Egregia menziesii 100 225 Gelidium spp. 15 190 Endocladia muricata 50 Corallines-crustose 20 Corallines-articulated 700 120 450 240 20 35 50 150 30 15 15 580 80 50 150 750 375 180 100 50 80 550 900 40 230 130 50 50 153 50 1500 200 100 300 80 230 300 550 32. Population Distribution V Description of the area: 4-5 feet above lower low water few plants, rocky direct sun splashed at high tide horizontal Area Covered (cm /m /4) Location Species digartina papillata Hildenbrandia occidentalis Porphyra lanceolata 90 Pelvetiopsis limitata Endocladia muricata Corallines-crustose 15 30 20 20 4 10 10 20 S26 igure 1 Total number of species in each distribution square Ca Distribution type II III IV Location 10 8 11 10 3 10 10 9 11 12 13 11 12 8 10 12 16 14 12 17 15 20 10 2 2 2 3 3 Sa PPENDIX List of Species(full name) Additional Distributional information List of Species Agardhiella coulteri (Harvey) Setchell Alaria marginata Postels and Ruprecht Callithamnion pikeanum Harvey Cladophora spp. Codium fragile (Soringer) Hariot Coralline spp. articulated Coralline spp. crustose Cystoseira osmundacea (Menziés) C.A. Agardh Desmarestia herbacéa (Turner) Lamouroux Dictyoneurum californicum Ruprecht Egregia menziesii (Turner) Areschoug Endocladia muricata (Postels and Ruprecht) J.G. Agardh Enteromorpha spp. Fucus distichus (Gardner) Hollenberg and Abbott Gastroclonium coulteri (Harvey) Kylin "Gatgore"-green algae that grows on rocks everywhere Gelidium spp. Gigartina agardhii Setchell and Gardner Gigartina californica J.G. Agardh Gigartina canaliculata Harvey Gigartina corymbifera (Kutzing) J.G.Agardh Gigartina papillata (C.A.Agardh) J.G.Agardh Grateloupia sitchelli Kylin Heterochordaria abietina (Ruprecht) Setchell and Gardner Hildenbrandia occidentalis Setchell Iridaea spp. Laminaria sinclairii (Harvey) Farlow Laurencia spectablis Postels and Ruprecht Leathesia difformis (linnaeus) Areschoug Macrocystis pyrifera (linnaeus) C.A Agardh Microcladia spp. Pelvetia fastigiata (.G. Agardh DeToni Pelvetiopsis limitata (Setchell) Gardner Peysonnelia pacifica Kylin Porphyra lanceolata (Setchell and Hus) G.M. Smith Postelsia palmaeformis Ruprecht Prionitis andersonii Eaton Prionitis lanceolata Harve, Ralfsia pacifica Hollenberg (mss) Rhodoglossum affine (Harvey) Kylin Ulva lobata (kutzing) Setchell and Gardner 33 Population Distribution Description of the area: 4-5 feet above lower low water horizontal inshore direct sun Covered Area (cm Species 300 Endocladia muricata 300 1250 1000 375 Porphyra lanceolata 270 Fucus distichus Pelvetia fastigiata 50 475 Cladophora Ulva lobata 50 375 500 20 25 60 Gigartina papillata 25 Coralline-articulated 170 20 15 50 Hildenbrandia occidentalis 20 100 100 Gatgore 30 Prionitis lanceolata 20 Gelidium. Iridaeaisplendens 40 Gigantinalcanaliculata Gigartina agardhii Coralline-crustose Callithamnion pikeanum Egregianmenziesii Heterochordaria abietina Leathesia difformis Peyssonelia pacifica Total Number of Species 360 1250 50 1800 320 750 40 10 320 10 120 280 220 10 85 625 225 180 200 250 250 500 450 Population Distribution II Description of the area: 2-3 feet above lower low water vertical not facing waves Area Covered Location Species Endocladia muricata 175 Callithamnion pikeanum 100 Gelidium 40 2250 700 Coralline-crustose 75 30 140 750 Coralline-articulated 100 40 Porphyra lanceolata 1250 2250 Hildenbrandia occidentalis35 150 90 Iridaea splendens 50 70 200 120 Ulva lobata 80 Grateloupia setchelli 20 150 Gigartina papillata 1000 Fucusdistichus 2250 Egregialmenziesii Gigartina agardhii Rhodoglossum 625 Gatgore Peyssonelia pacifica Iridaea flaccida Agardhiella coulteri Leathesia difformis Pelvetia fastigiata Gigartina canaliculata 250 Alaria marginata Total Number of Species 250 750 100 125 6600 150 200 190 10 100 20 12 625 50 10 25 50 900 300 625 250 75 100 155 20 20 1500 1625 750 1250 500 40 80 250 150 370 150 3000 390 1( 830 625 450 375 600 13 12 32 Population Distribution III Description of the area: vertical 2-3 feet above lower low water inshore facing waves Area Covered (cm Location Species 2000 Endocladia muricata Iridaea splendens 150 35 Coralline-articulated 700 70 200 60 Coralline-crustose 70 Hildenbrandia occidentalis 300 20 Gelidium Ralfsia pacifica Fucus distichus Gigartina papillata Ulva lobata Porphyra lanceolata Heterochordaria abietina Cladophora Gigartina agardhii Gatgore Iridaea flaccida Callithamnion pikeanum Egregia menziesii 300 Agardhiella coulteri Leathesia difformis Rhodoglossum Pelvetia fastigiata Gigartina canaliculata Alaria marginata 1875 700 Prionitis lanceolata Microcladia 11 Total Number of Species 420 110 40 256 1750 400 30 200 10 10 100 700 1000 120 750 1000 1000 320 70 100 1500 100 625 90 30 30 320 100 100 200 50 750 100 100 40 360 60 300 3000 100 20 130 20 60 12 16 10 400 50 1125 275 140 275 120 200 120 60 10 750 240 14 Population Distribution IV Description of the area: 3-4 feet above lower low water heavily populated standing water direct sun near to wave action Area Covered (cm Location Species 190 Endocladia muricata 700 120 Coralline-articulated Gelidium 225 Hildenbrandia occidentalis 225 1250 50 Coralline-crustose 200 Gatgore 80 Fucus distichus Heterochordaria abietina 80 100 Egregia menziesii Gigartina papillata Leathesia difformis Ulva lobata Gastroclonium coulteri Iridaea splendens 100 Pelvetia fastigiata Gigartina agardhii Prionitis lanceolata 160 Cladophora Rhodoglossum Alaria marginata Microcladia Porphyra lanceolata 450 Grateloupia setchell Callithamnion pikeanum Postelsia palmaeformis Gigartina canaliculata Agardhiella coulteri Total Number of Species 450 240 100 220 20 50 170 1250 600 180 160 240 50 170 30 20 10 60 12 15 580 30 400 15 100 15 100 250 150 1200 50 35 10 20 50 130 50 1500 40 230 275 220 50 1525 15 50 10 900 100 15 10 80 550 1000 50 100 375 180 20 750 150 150 50 50 50 80 20 230 550 80 120 300 330 300 100 100 200 Population Distributiony Description of the area: 4-5 feet above lower low water few plants, rocky direct sun splashed at high tide horizontal Area Covered (cm Location Species digartina papillata Hildenbrandia occidentalis Porphyra lanceolata Pelvetiopsis limitata Endocladia muricata Coralline-crustose Total Number of Species 30 35