17 THE ACTIVITY PATTERN OF Tegula funebrali Mark W. Howe Hopkins Marine Station, Stanford University s seat fp The activity pattern of the black turban snail, Tegula funebralis (A. Adams, 1851) at Pacific Grove, California, is the subject of this article. Field studies were carried out to follow the locomotory and feeding activities of individuals of T. funebralis to determine how much of each animal's time was spent in each of these activities, and when and under what environmental conditions they occurred. Field observations of Tegula funebralis in this study were carried out in the tide pools on the N.E. side of lussel Point at Pacific Grove, California. The intertidal substrate herd is composed of granite cutcroppings and boulders; depressions are filled with coarse sand and shell fragments. The vegetation is primarily Gigartina (Agardhii and leptorhynchos), Peyssonnelia, Ralfsia, and a thin green algal film. Any heavy surf is broken up before it reaches this area by an offshore granite reef. Inshore ocean temperatures varied from aboût 11 to 14°0 during the period of observation, 25 April to 1 June, 1963. Four individual Tegula funebralis were chosen to followy in detail. Their sizes are given below. Specimen A—greatest basal diameter- 19m 19mm Spedimen B 21mm Specimen C 18m Specimen D Each specimen was marked for better visibility by(a) cementing a distinctive geometric 2 Mark Howe figure made from reflector tape to the shell and (b) by cementing a wire eyelet to the shell and attaching a 10 cm length of brightly colored thread to this, making the animal visible even though buried among algae, rocks, or other snails. These animals were replaced in the same tide pool area from which they had been collected be at approzimately 3 feet above mean lower low water, and observed for periods at frequent intervals. In organizing the notes taken from these observations, a system for graphing the information was developed so that important relationships could be more readily seen. Fig. 1, which covers a complete tidal cycle, is representative of my findings. It represents a systematic diary of the activities of the animals which could be followed, and of some of the environmental conditions. Data are recorded as follows. persod (à) The exact times of actual observation, are shown by a black line at the top of Fig. 1. (b) Meteorological conditions and surf indicate the approximate conditions of sky and sea during the period of the observation. (c) The symbols for locomotion indicate occurence of this activity. When at the beginning of a peroid of observation a given animal occupied a different position than that noted at the end of the previous observation, locomotion was indicated as present but not observed. (d) Feeding activity is also indicated in Fig. 1. It was never easy to det dack fae exactly when the animals were feeding and when they were not. Preliminary studies of animals in laboratory aquaria revealed several different techniques for determining presence or absence of radular motion and these were used as indications of feeding s seen in the field. When ebserving Tegula funebralis from above, the mouth area fan-be- viewed observecto spreadswith each stroke of the radula. If ebserving from the side and a profile of the mouth area is exposed, the movements of the buccal mass produce a indentation anteriorly on the head at each stroke. Where conditions did not permit observing the above actions I sometimes had to depend on the rhythmic halting loco¬ motion or rhythmic motion of the shell characteristic of a feeding animal, of on rhythmic motion of an algal substrate as an indication. (e) The position symbols show the relative position of the snail on the rock substrate. (f) The degree of immersion symbols show whether the smail was continuously submerged, awash, or out of water entirely. (g) Height of tide is a graphis representation of the tidal curve, based on tide table predictions and application of the one-quarter-one-tenth rule. er One of the first relationships noted was the lafge numbers of animals on submerged horizontal rock surfaces at high tide. Fig. 2 shows the behavior of three individuals, indicating their positions onhroeks under various conditions. When out of water they tend to occupy such sheltered spots as under rocks and in crevids, which Mad offer some protection from dessication. As shown in Fig. 2, no animals out of water 17 Mart Houe wick were found on the tops of rocks, and the single individual whe remained on a vertical surface was well protedted by Gigartina. However, many snails start to climb vertical rock faces as soon as the incoming tide submerges them. They follow the water level,and hence tend to cluster on the tops of rocks submerged at high tide,and in belts around the sides of rocks not wholly submerged at hégh tide. This tendency to climb with the tide is borne out by the increasing percentage of time spent on Sa 8 vertical and horizontal rock surfaces at times when therare submerged. They then nese follow the recegding water level badk down the sides of the rocks when they are awash at the ebb of the tide. This(Characteristic)massing of Tegula funebralis on submerged horizontal surfaces is less(tæ during the day than at night, however. Fig. 3 shows the results of an experiment, conducted in a cloudy day, in a greenhouse aquarium. This aquarium was Eleven provided with running seawater and held at a constant water level. -11 animals (3 large, 2 medium, and 3 small and 3 minute) were individually marked and placed in the aquarium. Observations were made at half hour intervals and displacement ut or down since the previous observation was noted by an upward or downward arrow in Fg.2 the field noted. Each bar represents the sum of the number of times animals were o observed to have moved upward and downward in a 2 hour period. There appears to be a correlation between time of day and the upward vs. downward movements of T. funebralis. The figure indicates a slight but definite downward activity about Hart fowe 5 sunrise and a corresponding upward activity about sunset. This situation is nobce borne out by observations in the field where one can observe large numbers of the animals on the submerged horizontal surfaces at night but fewer during the day. On the sample field record (fig. 1) it is apparent that feeding took place only when the animals were wet. These and all other field observations on feeding are shown graphically in Fig. 4, which records the percentage of each animal's total feeding time which was spent under each degree of immersion. Most of the time when the animals are feeding they aressubmerged. About one third of feeding activity occurs while the animals are awash. Feeding activity out of water onlu occurs immediately following the awash state and is negligible. waths In general, in the low tide situations typical for the individuals followede of any kind no activity/was present. In contrast, during much of the time the animals were submerged or amash, they were either moving or feeding or both. There is an important exception to the above generalizations, however. This occurs in the special case of the high spring tide. Fig. 5 shows field observations nade during two spring tides, both over 5 feet. In each case feeding and other activity ceased during the peak of the tide. The only locomotion shown occurred when the snail was washed loose and forced to move to regain the rock. C Mark Howe Captions for figures Fig.1 Behavior of Tegula funebralis under natural conditikns. The symbols at the bottom of the figure represent environmental situations/ and the position or redponses of the animals, Fig. 2. Position of T. funebralis under various degrees of immersion. Fig. 3. Movements of T. funebralis in an open greenhouse aquarium. Numbers at top indicate 2-hour intervals startigg from midnight. Fig. 4. Percent of the total time spent in ffeding which occurred while the animals were submerged, awash, and exposed. Fig.5. Activities during two spring tides. Symbols as in Fig.1. 120 . Fig. I Legends Cloudy No activity Sun Activity present 9 Moon (half) Probably feeding Denotes bright Locomotion but Calm surf L not observed a Medium surf On top of rock 1 Heavy surf Jo On side of rock 2hoo o400 2000 1800 2200 0200 0600 Observation Meteorological o Conditions errerever Surf A - — Locomotion Feeding I1 To- 6 1o+ Position e 18 Aoongggnraree Degree of 00 Boodovat Immersion Corneoo 2 0800 In crevice Under rock Submerged 590 Awash 802 Out of water 1200 1600 1000 1400 1800 6 eee geggezevees eeeogred aeasgerorb tfft are M. Hone Number animal moving Number Of animals moving down Top rock P of rock Cre¬ vice Under rock A B A E Out of wate A E 8 8 8 E 122 Se of vater oatl fesdin 2400 2000 0200 2200 T e o Feeding 0200 1800 2200 0100 200 ——— -— o rvation Meteorological 8 a 111 1 Conditions mnn Surf Locomotion HE D Feeding Kr2D Position Degree of Immersion Howe