ABSTRACI The submersible ROV of the Monterey Bay Aquarium Research Institute provided video footage of crab-anemone interactions and was used to document a symbiotic relationship. Chorilia longipes and ++. Lorannnorpnde appear to innavit a common depurt range and pattern of distribution in the Monterey Canyon, and 818 of the corallimorphs veiwed were hosts to crabs. Chorilia longipes were found between 298 meters and 650 meters, and the corallimorph was seen between the depths of 306 and 506 meters. The corallimorph appeared in highest concentration at North Wall and were virtually absent south of Point Joe. Video vations suggest that corallimorph reproduce b both asexually and sexually Ihe crabs gains food and possible protection from their host anemones, and thus the relationship may be best desribed as commensalism. INTRODUCTION Many symbiotic partnerships have been described for species of fish, shrimp, and crabs with sea anemones. Many case in tropical coral reeis have been reported, including studies of an inachid crab with an - 41 imity for an anernone (Diesel, 1988, Weinbauer, Nussbaumer, Patzper, 1981), and an anemone which attaches to the shell of a hermit crab (Brooks, 1988). Small amphiprian fishes studied in the South Pacific and ian Oceans of a size to be considered prey of tropical sea anemones, but somehow acclimate to their host's nemotocysts (Mariscal, 1966). Studjes of symbionts in the temperate coastal seas are rarer, but some relatioriships have been documented. A small benthic fish may be found resting on Corynactis (Miller, 1989) and hermit crabs (Broughton, 1975; Tates, 1980) of Montery Bay have been observed in association with +- pleura However, nothing has been reported on deep sea benthic syrbionts. The Monterey Bay Aquarium Research Institute (MBARI) studies the fauna on the Monterey canyon wall using a submersible Remotely Operated Vehicle (ROV), and thereby provided a data base for the study of a leep sea decapod associated with an anemone host. This study investigates the depth range, pattern of distribution, and occurance of the symbiotic relationship of the corallimorph anemone and a smaller spider crab in the Monterey Canyon. Video footage provides the opportunity to study these symbionts in their natural habitat, and to make comparisons with observations in captivity. The purpose of this study was erunder ain ab tanding of the nature of these deep sea organisms + - and their interactive relationship. MATERIALS and METHODS The MBARI's ROV had video taped 46 dives in the Monterey Canyon between the dates of March 20,1989, and May 13, 1991. These were used to provide data for this study of the symbiotic relationship of a deep sea i Jecapod and anemone. The eleven signts and their locations are as follows: North wall (36 degrees 48' 30“ N, 122 degrees 5' 20“ W), Soquel Canyon (36 rees 49' N, 121 degrees 59' W), Meander (36 degrees 43' 06“ N, 121 decrees 9 30 W), Monterey Canyon Head, Canyon Wall C4-C5 (36 degrees 42' H, 122 degrees 00' W), Anthomastus Ridge, Point Joe (36 degrees 36 45"N, 122 degrees 00 15" W), Carmel Bay (36 degrees 32.2'N, 122 er egrees 58' 00" W), Cypress Point (36 degrees 42' N, 122 degrees 00' w), and Point Lobos. The videotapes are annotated and entered in the data base with physical factors and navigational data. The video footage of crab and anemone interactions from the 46 dives was reviewed and the depth of each sighting was noted. Lynn Lewis of MBARI provided data regarding the depth and the time spent on the canyon wall during each of these dives. By watching the tapes, it was determined if a crab was visible, the position of the crab, and any interaction which may tell more about the relationship je crab and anemone. The crabs are often covered with sediment betweent ze than their coelentarate hosts, and thus are not and are smaller in: asny seen with the ROV unless the camera is paused and focused closely nthecorallimerph on the canyon wall. The canyon wall C4-CS site was viewed for 2051 minutes between he denthe meters and 511 meters, the results from this sight. 9 particularily the dive on May 6, 1991, provided most of the data pertaining to the symbiotic relationship of the Chorilia crab and Corallimorph. The data base was also searched to provide information about every crab sighted during the 46 dives to learn of its other associations. It was occassionally seen with another coelentarate, the cerianthid, and therefore video footage of certanthids was also reviewed for symbiotic associations with the crab, and the depth range of the three species was noted To further investigate the relationship, six Chorilia and three -....:. Scarprerphs were observed at the Monterey Bay Aquar ium's research tenter. Two crabs and two corallimorphs were held for 35 days, one anerone and four crabs were collected May 6, 1991, and were held for 22 days The organisms were maintained in aquarja with recirculated water at 4-9 degrees celcius. The sea tables were kept in the dark. They were observed with ared filtered flash light or dim ambient light. The anemones and crabs were fed frozen krill every other day during the observations. The food was set on the face of the anemones and on the ocks next to the crabs. ile aptiity. Dw + D n ma au a the larvae were made using a camera lucida. Newly hatched larvae were oiaced on the tentacles of the anemone to determine if the anemone stung he larvae. A compound microscope was used to view larvae at 1000x in searchof the stinging nematocyst cells of the anemone. Atest to determine photreceptivity of the larvae was conducted on the second. third, and fourth days after one of the female crabs began to e her larvae. A five inch by six inch grid was drawn on the side of a one dallor clear plastic tub. The female crab sat next to her anemone on a rock. the larvae she had released were contained in this tub by a thin filter. Light was shined with a flashlight onto one 1" square for one i minute After the minute, esthnated counts were made of the number of larvae in each of the thirty square RESULTS dentification Daphne Fout in of the University of Kansas suggested that the raoroh anemone may be one of two species of the genus Sora Lorallimertrus reported in the South Pacific, both Corallimorphus rigidus - and Corailimorphus profundus have been described from preserved material. Four rows of tentacles were counted on the specimens in --... captivity, thus there is reason to believe this is the species Corallimorphus profundus which has been described as having tentacles in cycles of four. The spider crab was identified as Chorilia longipes with the key and description provided in Marine Decapods of California (Schmitt, 1921). The crab and anemone are conf ident ly recognized in video images when inspected closely. Sov Observation Archival video tapes from Monterey Canyon sites were viewed for a total 57Ol minutes and the total depth range analyzed was 31 meters to 511 meters. Table I lists the dive sites, the time spent on the canyon wall, and the minimum and maximum depths viewed on each dive. During the 8701 minutes on the canyon wall, 386 corallimorphs and —- ds were sighted. Table 2 lists the numbers of these terar enfarates from each site, and a calculation of the number of organisms en. seen per minute. Depth ranges. Table J lists the depth ranges for each organism. The corallimorphs were found within the depth range of 307 meters and 506 meters. The cerianthids were found within a shallower depth range of 67 meters and 434meters. The depth range of Chorilig was 299 metero to 506 metero. On a Floss Landing dive in October 1990, five to ten Chorilia were collected in hexactinellid sponges while dredging at 650 meter. - + Pattern of Distribution. Corallimorphs were commonly seen at North Wall, Soquel Canyon, the Meander sites, C4-C5, Anthomastus Ridge. There was only one seen on de wall at Point Joe. Corallimorphs were not seen at Point Lobos, the Monterey Canyon Head, Cypress Point or the Kelp study sites of Point Joe — 0.190 m, 357.840 m) and Carmel Bay (106.070 m, 446.500 m) at which + atriei uian the canyon wall were searched, they have been eeae sighted at these depths at other sites (Tables 1 & 2). he greatest populations of corallimorphs were seen at C4-C5 and Morth wall. Footage from tapes 10/26/89/11 and 10/26/89/12 show a patch of approximately 216 anemones between the depths of 351.430 and --- 00.010 meters at a North wall site. This dense population of anemones 4. -.:.- was disthictly different from other sightings. Table 5 compares number of solitary anerones to the number of anemones which appeared to be clonal, all anemones seen at the North wall patch were listed with the clonal anerone. The abundance of cerianthids is compared to corallimorphs in Figure Cerianthids were seen all sites except at Cypress Point. Ninty-five cerianthids were seen at the Pt. Joe site compared to only two sorallimerphs. They were not seen at depths beyond 434.040 m. Smsietic Belationshit. Corallimorohs were viewed closely at North wall, Soquel Canyon, -- th ade 2e, C4-C5, Anthomastus Ridge, and Point Joe (Table 4 and igure 2) Chorilia were seen with corallimorphs at each site except for the four corallimorphs viewed at Soquel canyon. Eighty-six percent of the 53 corallimorphs observed at C4-CS had crabs in association; 41 had one rab visible, and 13 had two crabs visible. Eight of the twelve corallimorphs at North wall were associated with crabs, and 1002 of the corallimorphs seen at the other three sights had one or two crabs. Fidure J shows th t 0 of the corallimorphs viewed had one crab visibie, 17.37 had 2 crabs visible, and 18.9% had no crabs visible. Thus, overall 31.17 of the ninety corallimorphs viewed closely had crabs in sociation. Chorilia were seen with other coelentarate hosts. At C4-C5, three cräbs were seen under cerianthids, two under duster anemones (Liponema), + two under Alliiomastus, and one in a shaggy sponge. At Point Joe, one Chorilia was seen in association with a cerianthid and another was seen in a folded sponge. One Chorilia at Anthomastus ridge was seen with an Anthomastus Beproductive Activity of the Corallimorph Corallimorphs were seen in strings of graded sizes suggesting they might result from asexual reproduction. On footage at time code 10/26789/11, 00.29.45.00, a corallimorph of about 5 inches in diameter abbeared to be dividing in half- the split occured at the mouth. Gilbert van ne Monterey Bay Aquar ium has some observat ion suggesting Dyhheusenatt incibient asexual reproduction in captive anemones. Footage from 05/06/91/07, 02.19.15.00, shows a corallimorph eleasing gametes or larvae out of its mouth. The epidermis of the corallimorph is a transparent enough to observe the ripe gonads. The gonads were seen in bands associated with the margins of mesentaries. Association in captivity and in the wild Video footage and observations in the aquaria showed crabs situated close to the anemone. There were two common positions of Chorilia ciosely associated with the anemone. The crab may have ito ventral surface touching the anemones column, the middle two pairs of legs nearly wrabbed around the column, and the long, slender, front legs touching the . tob of the colurn and tentacles. The other common position is with its —...-- J0sar surace to the column and facing out. A crab would usually touch the column with its hind legs, the tentacles of the anemone would hang down and appear to touch its back. Video footage showed how a crab could be corpletely hidden away under the draping tentacles of a corallimorph. A female in captivity was seen poking a tentacle with her leg, and on one occasion she appeared to repeat a motion of stroking a tentacle. In the Montetrey Bay Aquarium, it was observed that the white club-tips of the corallimorph would sometimes stick to the legs of the crabs. Iwo cabs from different anemones were placed on tentacles of an anernone. The smallest crab struggled to climb off, but was stuck and had to be removed. A larger crab managed remove itself. Both crabs reacted duickly to escape off the sticky tentacles. A crab collected at 650 meters in association with a hexactinellid sponge was placed in a tub with a corallimorph. This crab moved to the side of the rock when placed in the tank. In four minutes it approached the corallimorph and touched a tentacle twice with its front right claw; the club-tip stuck to its claw. The crab then walked away from the anemone and settled under the rock. After 15 minutes it had moved completely around the rock, and settled under the corallimorph. The crab picked up its egs and brushed the corallimorph, and the club-tips appeared to stick. The corallimorph did not appear to move it tentacles to touch the crab. At 17 minutes the crab was tucked up under the corallimorph with ak e eus touchind the column. The entire carapace was brushed by the ted under. The club-tips would stick but then let go. At 30 mmutes un ciab was stiff tucked under the corallimorph. At 34 minutes. the crab tugged to pull its leg away from a sticky club-tip. It appeared that the crab was tucked up on the rock next to the column, enough below the hanging tentacles so not to be stung. At 40 minutes the crab remained under the corallimorph. eeding The anemones and crabs were given large and small krill. When food was set on the face of a corallimorph, the mouth would open and the 4. ie acles would appear full rather than shriveled. Food was moved closer to the roouth and taken into the oral cavity, but regurgitated food was frequently found at the bottom of the tank. The anemone did not exhibit a feeding response to live brine shrimp. The shrimp would stick to the tentacles, but would frequently swim away. A corallimorph would fold a tentacle over a shrimp, but it did not bring the prey towards its mouth, or crack open its mouth. The crabs have been seen feeding on both the large and small krill. They would remove pieces from the rock and occasionally from an amemone. The crabs swung their claws to catch the swimming shrimp, and they ate shrimp that stuck to their carapace and legs. Foctage from 10/27/89/06, 1.58.25.00, shows a crab partially sittina on the face of an anemone, reaching its two claws into the oral ting of the corallimorph, and removing food. A female crab in captivity was also seen exhibiting this behavior, she used her front legs and claws ... .. to take food on the anerone while club-tips moved and touched her front eds ine crab was seen pinching a tentacle. When the club tips stuck to ner leus. she did not pull away quickly like a reflex- she seemed to ... Coneinde with her business of getting food. -- e ion in captivit nobservations made once or twice every day for fourteen consecutive days, an adult female Chorilia was always seen on the rock next to an anemone. She was seen facing it 7 days, facing outward 8 dayo and sittina sideways next to it on one occasion. This crab was seen next to her anemone in all 21 observations made during her 25 days in the holdind aduaria. sinan male crab spent the first four days on the rock near the anemone, and on one occasion it was seen touching the base of the anemone. In 12 observations made during the following 14 days it was seen under a rock, and it spent the final 4 observed days in the corner of the tank A collected adult female was seen facing the small anemone with her front two legs up touching the top of the column at the edge of the tentacles. She remained there for 6 days, but was removed from the aquar ium when she began to release larvae. A large male crab and corallimorph collected were observed 13 of I5 days in captivity. The crab was seen on the rock either up close facing the anemone or within about two inches of it. A female was collected iis day and also placed in this tank for behavioral observations. She spent her first five days on the rock, facing the anemone; she was on the opposite side of the anemone from the large male crab. She released her larvae in the corner of the tank. She was placed in the other tank with the all anemone and was seen associated with it for 4 of 4 days of vation. The fourth crab collected that day appeared to be a small female. It was seen on the rock approaching the large male next to the anemone. The large male used its claw to pinch the leg of the small crab which immediately backed away. This crab did not seem to establish a relationship with one anemone. On various observations it was seen in the ++ +- corner of the tanm, on the rock, hidden under the rock, and under the anerone. Larvae The three crabs released their larvae between the dates of May 10 and May 16. One crab was observed leaning back, balanced on her four hind eds. Her front two legs were reached up and outward and the other pair were extended out to her sides. Her front legs were seen touching the edge of the corallimorph, and her claws were folded down. She raised her abdomen to contract the egg sac and O,1,2, or 3 larvae were seen to come out at the top. The carapace of the newly hatched zoea was approximately one millimeter, with an abdomen of about one mm in length. The crabs pumped eggs out of their egg sacs for three to five days. Drawings were made of the surviving larvae on several days after 1-- - natching. After two weeks, the dorsal spine on surviving larvae, shortened inendt h. Notable characteristics and measurements are included on the drawings (Figures 3 and 4). The larvae stuck to the tentacles of the anerone, but like the brine shrimp, were not injested. Test for photo receptivity showed that the larvae are attracted to light. Zoea were most concentrated in grid squares where light is shined (Figure 5). Larvae swam toward the light then dropped down. DISCUSSION ROV Observation Depthan de corallimorph, cerianthid, and Chorilia longipes exist in +. thids were found at 67 meters which ing deurrandes. The cerian ——— 5 2rmheters shallower than first crabs seen. Its range extends to 434 .5 on e MEARI archival videos. Only four sites had dives going eeber than 434 meters. The depth range of the Chorilia was seen by the BOV as 299 m to 506 m which closely parallels the depth range of the - Corallimoruh (307 m to 506m). The crabs collected at 650 meters give proof that Cherilia lives deeper than the 511 meter maxium dive made by the ROV. The crab collected at 650 metero positioned itself under a corallirorph within 15 minutes. The larger tether of the ROV will soon bermit establishmnet of the lower depth range of the anemone and crab. . n of Distribution a Coraimorphs were seen in greatest abundance at North Wall. They were also sighted at Soquel Canyon, the Meander site, Anthomastus Ridge, and Ca-C5 Corallimorphs were rare or absent from Pt. Joe and south. The reatest concentration was seen at the North Wall where many corallimorphs appeared to be clonal, moving south to C4-C5 and thornastus Ridge, most corallimorphs sighted were solitary. Only one corallimorph was sighted at Pt. Joe, and none were seen further south. Corallimorphs were not sighted at the shallower depths studied at the Monterey Canyon head. At Pt. Joe and Carmel Bay, corallimorphs were not seen on the level sites of the Kelp study dives. This follows the battern of nearly no corallimorphs south of Pt. Joe, but it may also suggest mat die corallimorphs prefer the rocky sloped habitat of the canyon wall. The dive at Pt. Lobos was shallower than the upper depth range of the corallirorph, but Cypress Point was viewed within the appropriate range and none were seen. Further study using transects of the different dives tes may aive more insight on the possible significance of this ** vacion. Environmental conditions such as oxygen content, terberature. ssure, or rutrients, as well as reproductive capabilities, may contribute to a patchy distribution. Cerianthids were seen at all sights except Cypress Point. They were found in abundance on the canyon floors of Pt. Joe and Carmel Bay. These tube dwelling coelentarates appear to find the both the sloped rocky walls and smoother floors as suitable habitats. Again, it would be beneficial to make a more detailed analysis of the significance of their distribution in he Monterey Bay Canyon. Symbiotic Relationship. The percentages of corallimorphs seen with crabs emphasizes their strong symbiotic relationship. The overall value of 818 corallimorphs viewed with Chorilia is probably a low estimate. The crabs can hide under the tentacles of the anemones, and make it difficult to determine whether a crab is actually present. It was interesting to note that no crabs were seen at Soquel Canyon, although only four corallimorph were viewed closely, it is possible that Chorilia do not inhabitat that area of the canyon. Cherilia were occasonally seen with other host organisms including four cerianthids, one folded sponge, two under duster anemones, three under Anthomastus, one shaggy sponge, and a few hexactinellid sponget The crabs were well hidden, which supports a hypothe sis that protection fror predators is one of the reasons for the symbiosis. Smaller crabs were seen on the Anthomastus suggesting that the younger crabs may find hosts other than the corallimorphs until further development. The smallest crab in the aquaria was unable to climb off a corallimorph when set on its tentacles, the corallimorphs in the wild may have the capability of capturing small crabs for consumption. Beproductive Activity of the Corallimorph: The corallimorph which appears dividing at a deep furrow through ne mouth suggests that these organisms can divide asexually. They were so seen in chains decreasing in size, as though new organisms had bud om a barent. The footage of a corallimorph releasing gametes or larvae sugdests sexual reproduction. issogiation seen in captivity and in the wild Behavioral observations made in the aquar ia resembled scenes in the deo footade. Chorilia did not appear to exhibit any modified behavior caused by their new environment. Unfortunately, the corallimorphs have not fared so well in captivity. Two of the corallimorphs observed during this study resembled those in the wild; their tentacles were full and extended gracefully. Tentacles of the third anemone were stubby and bal ely extended off its race, other corallimorphs in captivity in the onterey Bay Aquar iurn's deep sea tanks have a shriveled appearance bert Van Dykhuizen suggested that the corallimorphs have not been diven an apppropriate diet. The regurgitated food sugge ts that the anemones are either overfed, or are starving by not digesting the food. Further research of these symbionts should first include determination of edieto e corallimorphs, under standing their diet may help maintain these organisms in captivity, or could suggest that other factors have caused their decrease in size. Feeding Crabs were seen taking food out of the mouth of a corallimorph on tape and in a tank, and thereby benefiting from a free meal. Small particles and occasional larger prey can be caught by the sticky mucus and stinging nematocysts of an anemone's tentacles; the crabs have shown that hey are interested and can feed off the food of these corallimorphs. This may support a hypothesis that food gives the crab another benefit from a ostanernone e All three female crabs released zoea between the dates May 10 and May 16, 1991. Two caus were collected from Monterey bay on May 6 1991. The third crab to release her larvae was in captivity for over a month, therefore it appears that captivity has not interfered with the normal reproductive cycle. Zoea maintained in a tank with a constant water current survived for aminimum of 21 days. Drawings of larvae show five segments of the abdornen and then the telson. The four leg appendages had four setae each throuahout the observation time. Counting the setae is a method used to stade brachyuran zoeae, additional setae would be expected to develop, but were not seen on the specimens observed. The shortening of the dorsal pine may be evidence that some of the larvae were developing. Toea larvae feed on planktonic organisms. (Warner,136) Positive phototaxis may direct the larvae up towards this food source. The three to five day old zoea were attracted to the light shined on the tank. They sensed the direction of the light and were most concentrated in grid squares at and below the source. When they swam to the light, it appeared that they hit the side of the tank then fell to the floor, but when light wat shined at the surface of the water, they swam up to it and then descended. The arids in Figure..*show that when the light was shined in an upper square, the concentration of larvae is high in the entire column. When licht was shined at a bottom square, the concentration was highest at the lowest square, but was decreased up above. Warner (1977) states that the direction of swimming seems to involve responses to both light and gravity at nten etion of observations Chorilia longipes and Corallimorphus appear to inhabit a common depthrange and pattern of distribution, and eighty percent of the corallimorphs are hosts to crabs. Of the 101 Chorilia sighted, 89 were seen with corallimorphs and four were seen with cerianthids. This may + ++ sugdest that the prefered host of Chorilia is Corallimorphus The symbiotic relationship between the crabs and their —-- elentarate hosts may be desribed as "commensalism in which one the her harmed or benefited" (Märiscal, hefited and the other n 1369). Observations suggest that the crab gains protection from outside predators when hidden under the anemone. The crabs can be extremly difficult to see when they are associated with the anemone. Predators of the crab may not see a crab hidden under an anemone, or may avoid contact with the stinging nematocysts of the club tipped tentacles. It has also ata Mnoted tiat tiie ciaus occasional feed on the food collected by the corallimorph, and thereby gain a nutritional benefit through this symbiosis. From this study, the corallimorph has not appeared to gain or lose in this relationship, further study may indicate otherwise. Ihe crabs may receive another possible benefit which deserves fürther study. Small live brine shrimp were seen swimming into the crabs and sticking. The crabs removed the brine shrimp from their legs and carapace, and then fed. Two crabs waved their claws in the water to wash the sticky shrimp off. It would be interesting to determine what made the shrimp stick to the crab. It could be the canyon wall sediment on the crabs, mucus from the corallimorph, the nature of the brine shrimp, something secreted by the crab, or a combination of these. Observations of the crabs with the corallimorph showed a short. any, acclimation process. Crabs set on a rock next to an anemone in captivity were seen touching the anemone within minutes. The crab from 650 meters was seen under a corallimorph within 15 minutes of entering ne tank. Ihe club tips obviously stuck to this crab, it was seen pulling its ed away from one of the anemone's sticky club-tipped tentacles. This howsthat at leasteariy in the relationship,t e corallimorph will sting -oub but this does not appear to prevent the crab from establishing a eationsni. Studies of hermit crabs suggest that the crabs become coated with mucus and may thereby not be recognized by the host anemone. The eurface of Chorilia could be investigated in future studies of these symbionts, it would be interesting to learn if the legs and/or carapace of an associated crab has a mucus coat or nematocysts on its surface. A detailed behavioral study may reveal an acclimation process in which either the crab or corallimorph adjusts to a new partner. Future studles of deep-sea organisms can only benefit from the opportunity provided by MEARI to complement experimental conditions with a of view the live activity of the canyon walls. ACKNOWLEDGEMENTS dlike to thank Lynn Lewis of MBARI for her assistance with the data base (and for her patience when we invaded the video room). Thanks to Chris Harrold, Gilbert Van Dykhuizen, Sue Lisen, Karen Light and Andy of the Honterey Bay Aquar ium for their interest and contributions to this project. Thank you to my roommates, Kirsten and Edith, for their support and good humor throughout the quarter. And THANK YOU Chuck Baxter, for the insightful questions and suggestions which brought this project all todether. RATURE CIIE Brooks, W.R. 1988. The Influence of the Location and Abundance of the Sea Anemone Calliactis tricolor (Le Sueur) in Protecting Hermit Crabs from Octopus Predators. Journal of Exp. Biol. Ecol. 116: 15-21. Broughton, P.S. 1975. The Symbiotic Relationship between the Hermit crab Pagurus Samuelis (Anthropoda: Decapoda) and the Sea Anemones Antnopleura Elegantissima and A. xanthogrammica (Cnidaria. actinaria) Student paper, Stanford University. Diesel, R. 1988. Male-female Association in the spider crab Anachus chatagium the influence of female reproductive stage and size. Journal of Crustacean Biology 8. 63-69. burney. E. Larvae of Decapod Crustacea. Engelman, Whelden, and Wesley, Lid. London. LubbOCk, R. 1979. Chemical Recognition and Nematocyte Excitation in a Sea Anemone. Journal Exp. Biol. 83. 283-292. 7--.- -. Fariscal, R. N. 1966. A Field Study of the Symbiotic Association of the Fishes and Sea Anemones. Doctoral Dissertation. University of California, Berkeley. Hariscal, R. N. 1969. Experimental Studies on the Protection of Anemone Fishes from Sea Anemones. Pp. 283 5. /: Cheng, T. C., Aspects of the Biology of Symbiosis. University Park Press: Baltimore, MD. Miller, S. 1989. The Association Process between Fish and Anemones in Monterey Bay. Student paper, Stanford University. Schraitt, W. L. 1921. The Marine Decapod Crustacea of California. Univ. of California Publications in Zoology. 209 pp. Warner. G. F. 1977. The Biology of Crabs. Van Nostrand Reinhold Company. HEw Tork, H. Weinbauer, et al. 1982. Studies on the Relationship between /hachus - malangrumFabricus (aiidae) and Anemonia sulcata Pennant in .... tiien datul ai Environment, Marine Ecology Weymouth, F. W 1910. Synopsis of the True Crabs (Brachyura) of Monterey Eay California. Stanford Univ. Press. Stanford, CA. Tates. G. D. 1980. A Symbiotic Relation between Intertidal Hermit Crabs and Sea Anemones. Student paper, Stanford University. EIGURES Figure 1. Compares the number of corallimorph anemones to the number of the cerianthids at each sight. Figure 2. Column graph representing the six sites where corallimorphs and Chorilia were sighted. The last column of each set represents the total for all sights. The first set represents the number of corallimorphs veiwed closely. The second set represents the number of crabs sighted with these corallimorphs. The third set shows the purober of corallimorphs with no crab visible. Figure 3. Pie grach showing the percent of ninety corallimorphs with one, two, and no crabs visible. Fiaure 4 Larvae between ages of 3 and 23 days drawn with camera lucida Figure 5. Body parts of larvae. Figure 6. Test for photo receptivity. First experiment shined light in a one inch square in the upper corner of the tank. The second experiment had light shining at a lower square. The third experiment represents ared light shined eight inches away from the tank, and the fourth experiment was conducted when no light was shined. Figure 300 0- Comparing the abundance of corallimorphs versus cerianthids at each ROV site corallimorph cerianthid E Dive sites 1. C4-C5 7. Anthomastus Ridge 2. Point Joe 8. Soquel Canyon 3. Carmel Bay 9. Mont. Canyon Head 4. North Wall 10. Cypress Point 5. South Meander 11. Point Joe 6. Meander k - . MEDSEEE 8 89 8 vS Emmumum u 2 Figure 4 LARVAE cohe logjes kete hag k 20 ae 3-12 dang Cealela loss ined mkto 3-7days old selson en Se d -—- A — ral Imm Ciha lohpe Belered Man- 13 12 Ha od W O 2 CHORILIA LONGIPES 2123 dau do uroine Figure 5 Components S me 400 ues 15-17 dags Gociso langipes 1S-17 daqs nouit area vsiblé af CHORILIA LONGIRS - 57dage od Abdomen S — N S 5-21 chorilia lonzipes 5. 7 daus ol4 Telson — S aa J ox Ed of 166 Figure 6 O 00 50 LH LCHT 100 O 00 50 30 0 30 20 RED 20 6 0 O 0 0 70 O O 0 0 10 50 wad 20 0 1 40 10 test for photoreceptivity 0 O 8 200 00 L EST Loh 80 50 50 30 0 20 TESI No 5 20 0 0 0 10 10 10 0 10 50 29 O O 4 O OMIN AETER TEST 2 6 0 0 0 0 5 0 /0 0 5 10 10 20 50 50 50 50 TEST . * — — — SONN. a o to aaaga- — 0 + —i U — — altutaanom- — - - —. — C E. 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