Robb Diet of Cyanoplax page 2 INTRODUCTION Although chitons are abundant along California shores, studies of their food and feeding have been limited largely to species of Mopalia (e.g. Barnawell, 1960; Boolootian, 1964). Placiphorella (see McLean, 1962), and Cryptochiton (Macginitie and MacGinitie, 1968). The purpose of this study was to examine the diet of the chiton Cyanoplax hartwegii (Carpenter, 1855) in different habitats. I also include a few notes on the slit formula of the plates of C. hartwegii. Intertidal Habitats of Cyanoplax hartwegii. Cyanoplax hartwegii on the Monterey Peninsula, California, is found in the mid and upper intertidal zones. Three general habitats where large numbers of C. hartwegii were present were studied. First, at approximately the +2.0 ft. tide level, C. hartwegii was most common underneath the brown alga Pelvetia fastigiata (J. G. Agardh) DeToni on otherwise nearly bare rock in an area of low wave action. Here Pelvetia covered approximately 70% of the rock area, providing a shady and moist environment even during daylight hours and at low tide. Because of shading by algae and by other rocks, only about 5% of the rock was exposed to sunlight at low tide. Pelvetia was by far the dominant alga; small amounts of Hildenbrandia occidentalis Setchell, Petrocelis franciscana Setchell and Gardner, Gigartina papillata (C.A. Agardh) J. G. Agardh, Fucus distichus Linnaeus, and crustose corallines were also RoBb Diet of Cyanoplax page 4 using Smith (1944) and were verified by Dr. I. A. Abbott. The digestive diverticulum was not extensively examined since several dissections yielded only blue green algae and yeast cells. Specimens of Cyanoplax hartwegii were identified as such on the basis of size, color, shape, insertion plate slit formula, and comparison with museum specimens of C. hartwegii and Cyanoplax dentiens (Gould, 1846) identified by Allyn G. Smith and kindly provided by the California Academy of Sciences. Average insertion plate slit formulas (11-1-11 for C. hartwegii, 11-1-10 for C. dentiens) proved unreliable for identification. In specimens conforming to C. hartwegii in all other respects, numbers of slits ranged 9-12 in the head valve and 8-11 in the tail valve (Fig. 1). The formula 11-1-10 occurred most often, which is the slit formula given for C. dentiens. SSULTS The results of the gut content analyses of 65 Cyanoplax hartwegii from the various habitats are shown in Figures 2 and 3. Since Pelvetia made up 70% of the plant cover of the Pelvetia habitat, it is not surprising that the 18 Cyanoplax hartwegii taken here ate large amounts of this alga. Pelvetia occurred in 89% of the digestive tracts and comprised the bulk Robb Diet of Cyanoplax page 5 of the material in each individual. Hildenbrandia occidentalis, and Petrocelis franciscana were found in much smaller quantities in many individuals. In the crevice habitat where Pelvetia was absent, Cyanoplax hartwegii ate moderate amounts of Hildenbrandia, Petrocelis, Endocladia, and Cladophora spp. The relative quantities of these algae in the digestive system varied in the 17 individuals examined, but for the population as a whole they occurred in roughly equal amounts in the total gut content. In the tidepool habitat the 30 Cyanoplax hartwegii examined displayed a more varied diet. Gigartina leptorhynchos appeared in many Cyanoplax here, but many other algae were present in small quantities, including crustose corallines. Even when Gigartina and crustose corallines were present in the Pelvetia habitat and crevice habitat, they were not eaten. Thus, it would appear that there is a preference for Pelvetia, Hildenbrandia, Petrocelis, Endocladia, and Cladophora spp. when these are present. In the gut of C. hartwegii, larger algal species (e.g. Pelvetia) and encrusting algae (e.g..Hildenbrandia) appear in very small pieces indicating feeding by rasping. However, algae with slender branches or highly dissected blades (e.g. Endocladia muricata, Gigartina leftorhynchos) appear in the gut much larger pieces, reminiscent of algal fragments found in the guts of Nuttallina californica (Reeve, 1847) by Robb Diet of Cyanoplax page 6 Nishi (1974), and suggestive of feeding by biting or tearing algal branches. SUMMARY 1. Stomach contents were examined in 65 specimens of Cyanoplax hartwegii taken from three different intertidal microhabitats near Monterey, California. 2. In the Pelvetia bed habitat, Pelvetia made up 80% of the total diet, while in the higher crevice habitat, the algae Hildenbrandia, Endocladia, Petrocelis, and Cladophora spp. comprised 90% of the diet and were eaten in relatively equal amounts. 3. In the high tidepool habitat, C. hartwegii ate a much wider variety of algae. Gigartina and Hildenbradia together made up 40% of the diet. Crustose corallines and Gigartina occurred in all habitats examined, but were eaten by Cyanoplax only in the tidepools. ACKNOWLEDGMENTS I would like to express my thanks to Dr. Isabella A. Abbott for technical help in all aspects of the gut content analysis and identification of algae. My appreciation cannot be measured. Her guidance in all areas of the study, and her insight and beauty as a sincere, warm human being made my time at Hopkins Marine Station an experience I will never forget. Robb Diet of Cyanoplax LITERATURE CITED Barnawell, Earl B. 1960. The carnivorous habit among the Polyplacophora. Veliger 2(4): 85-88, pl. 19. (1 Apr 1960) Boolootian, Richard A. 1964. On the growth, feeding, and reproduction in the chiton Mopalia muscosa of Santa Monica Bay. Helgolander wiss. Meersunters. 11: 186-199; 3 figs. (Dec 1964) McLean, James H. 1962. Feeding behavior of the chiton Placiphorella. Proc. Malacol. Soc. 35(1): 23-26, figs. 1 & 2. (Apr 1962) MacGinitie, G. E., and Nettie MacGinitie. 1968. Notes on Cryptochiton stelleri (Middendorf, 1846). Veliger 11(1): 59-61, pl. 6. Nishi, Rae. 1974. The diet and feeding habits of Nuttallina californica (Reeve, 1847) from two contrasting habitats in central California with notes on their natural histories. Veliger. page 7 Mark Robb page 2 Diet of Cyanoplax Figure Captions Figure 1. Number of Cyanoplax hartwegii with a specific slit formula. Figure 2. Ihe percentage of the total number of guts where a specific alga chitons from was found inthree habitats. Figure 3. The percent occurrence of the dominant algae in the total gut of chitons from three content habitats. SNenter op Cjono pos vitk sgerttie sit formila no S heao valve 23 o0 Figure 1 Mark Rebb Jo Occorrance of Léggool kabrtt 10 20 2040 90 601000 00 k —— 20 o 12070 ildenbvaudt 0% toeels 0% Endocladu 10% Gronta 10% Chach merghe 5% Crustosz cotalline 5% Palfse 10% oher Dmmant Akge in Qudt Crevice habites Pleha kabtt 30 40 9 10 70 80 40 100 20 2040 9 40 7 20 70 10 — 209 Reto 30% Hildenbande 10% Hildenbrandia —20% Rtoocelis 16% Rlocelis —20% Endoelade 20% Clado pheca 16% other 10% ater tigyre 3 Alga Hound o guts wilk Specifi o-age 70 8010 00 20 40 S60 70 80 40 100 O 2O304056 10 20 — — | — — | — | — — — — —.- --— Cladophora 29. e —- — Choetomohe elis fronciscara — — +- — tteromopha sp. Goia nnicate — nela Ehtoclodig eiaens ndionalis Setchell and Gardner enberg ard Abboff — — Gomontia polyrhiza dium 50 (Logerheim) Bornet and Hahaut g 3 idiaia minima (kgeli) Kglin UWe 2p. — bse cotallines — — Beudoubella consoeiato Setchell ard Gardrer kaaaaa- ina 7. — — Pelveha fastgase pysalis eneghie:) Drouct and Delg —— — — Lalssia 39. labria sp. — Leahese 2p. waria 59 — este -18 cide Coickie): Rhena habitar os0ad 5. 7 Crevice habitat oms — ge Sicules Tdz pool Fobtt 1- tote Ne Figure 2