Stevenson: Natural History of Kelletia Abstract The predatory gastropod, Kelletia kelletii, was studied in the lab and in the field to characterize its habitat characteristics, distribution patterns, activity patterns, and escape responses. The mean density of Kelletia was 0.32 m-2, and their depth range was 5.5 to 12 m. Snail sizes differed significantly between those found on rock and on sand: the mean size of snails found on rock was 78.4 mm, while the mean size for snails found on sand was 92.9 mm. Adult snails in the lab and the field bury themselves for up to 152 hours, punctuated with bursts of activity. There was no significant relationship between the percent time buried, the length of the buried period, the length of the active period, or the rate of movement, and size of the snails. No clear relationship existed between the activity pattern and foraging, and gut content analysis was inconclusive. Kelletia captured and consumed Tegula or Calliostoma ligatum in both the lab and the field. A large mating aggregation was found during the study. Snails were significantly larger within this aggregation. Kelletia exhibited no escape response to three sea stars and a crab. Stevenson: Natural History of Kelletia Introduction Kellet's whelk, Kelletia kelletii, is a large predatory neogastropod found in the subtidal zone to depths of 70 m. It grows up to 170mm in length and has a heavy, bulky, thick shell. It has a long, extensible proboscis used for feeding, which can be extended up to 3 times the length of its shell. The foot is very muscular, and is colored yellow with white speckles and black lines around the edge of the foot (Morris et. al., 1980). Kelletia was, until 1980, primarily a southern California species. In 1980, the first snails were discovered in the Monterey Bay area, and have since grown in abundance and can commonly be found in offshore reefs in the area (Herrlinger, 1981). Little is known of the behavior and life history of Kelletia, and what little work has been carried out has been performed in southern California. Its trophic association with Pisaster giganteus has been studied (Rosenthal, 1971), as has its reproductive biology (Rosenthal, 1970). This study examined the habitat of K. kelletii, its local distribution, and its activity patterns in the lab and field to determine if major differences exist between the expanding edge of the species' range and the central portion of its range. Materials and Methods The study was carried out at the Hopkins Marine Station of Stanford University in Pacific Grove, California during April and May 1994. Field studies were conducted in the Hopkins Marine Life Refuge offshore from the station (Fig. 1). Stevenson: Natural History of Kelletia Population Studies Random sampling was conducted to determine the population density of Kelletia in the Hopkins Marine Life Refuge. Random compass bearings and kick cycles (ranging from 5 to 50, approximately 1 meter per kick) were used to determine sampling locations. The type of substrate, depth, number, and size of the Kelletia were recorded for each 5-m2 circular quadrat. Quadrat counts were continued until the slope of mean versus number of quadrats approached zero (21 quadrats). Lab Activity Patterns A tank measuring 230 cm x 110 cm x 40 cm (water level maintained at 20 cm height) was used to track four Kelletia at a time to observe activity patterns. Their activity periods, distances and directions were recorded. Trial 1 was conducted for 65 hours, with four snails with distinct markings placed in different locations in the tank. A dead smelt was placed on the sand next to the inlet pipe approximately 27 hours after the start of the observation period. At the end of the observation period, the dead smelt was removed and the tank flushed with fresh seawater before the Trial 2 was begun. Trial 2 lasted for 216 hours. Four new snails were used and were tagged with waterproof epoxy. The snails were placed next to each other in the center of the tank. A dead smelt was added to the tank next to the inlet pipe after 124 hours. Trial 3 lasted 74 hours. In this case the dead smelt was in the tank to begin with and remained in the tank for the duration of the Stevenson: Natural History of Kelletia observation period. Again, four new snails with distinct markings were used and were placed in the center of the tank within 2 cm of each other. Field Activity Patterns Fifteen Kelletia were collected from the field on May 6, 1994. and were tagged with epoxy attached to their shells (Rosenthal, 1969). Numbers were etched into the epoxy to distinguish individuals. Bright yellow nylon strings approximately 10 cm long were attached in the epoxy tags to aid in relocation. These tagged snails were then taken to Location 1 (Fig. 1) and released at approximately 5:00 pm. The snails were then observed 18.5 hours later in the same vicinity. Headings and distances moved from the original drop point were measured with a compass and a tape measure to the nearest cm. A second group of twenty snails were collected and tagged according to the procedure outlined above, with an additional mark on the epoxy to distinguish the snails from the original tagged group. The snails were kept overnight and released at Location 1 (Fig. 1) the next morning. The snails were observed 1.5 and 4 hours later, and then again 73 hours after release. As in the first tagged group, headings and distances moved from the original drop point were measured in a similar fashion. Gut content analysis was performed on five snails: three collected from the sand, and two collected from rocks, to determine a relationship between activity patterns and foraging behavior. The snails ranged from 85 mm to 95 mm in length. Stevenson: Natural History of Kelletia Feeding Studies Six Kelletia were placed in a tank measuring 50 cm x 50 cm x 30 cm (water level at 28 cm) for five days to observe their feeding behavior. For the first three days, 5 Tegula montereyi, 5 Tegula pulligo, 5 Tegula brunnea, and 5 Calliostoma ligatum were placed in a tank with the Kelletia. On the fourth day, all the Tegula and Calliostoma were removed and replaced with 10 Tegula pulligo, 10 Tegula brunnea, and 5 Calliostoma ligatum. These were left for two days. On the sixth day, all non-Kelletia snails were removed. A second trial was run for two days with a new group of 5 Kelletia offered 5 T. montereyi, 5 T. brunnea, and 5 T. pulligo. After two days, all non-Kelletia snails were removed and small glass test tubes containing invertebrates were placed on their sides in the tank. The invertebrate specimens were collected from a vertical face of a subtidal boulder in the Hopkins Marine Life Refuge, and consisted of an oyster, a polychaete worm, a tunicate, a sponge, dead polychaete worm parts, a dead sipunculid worm, a living sipunculid worm, and a chiton. In addition to lab observations, Kelletia were examined in the field to determine whether they were feeding and, if possible, identify their prey. Mating Aggregation Observations On May 17, 1994, a mating aggregation of Kelletia was observed at Location 2 (Fig. 1). One week later the densities of snails in the aggregation was measured with a 1/16 m2 quadrat, placed 7 Stevenson: Natural History of Kelletia times at haphazardly selected points in the aggregation. The number of snails within each quadrat was recorded, and 68 snails were collected for size measurement. Escape Response Studies To assess whether habitat utilization may be influenced by interactions with predators, Kelletia escape responses to the sea stars Pycnopodia helianthoides, Pisaster brevispinus, and Pisaster giganteus, and to the crab, Cancer antennarius were examined. P. giganteus and Cancer occur primarily in rocky areas, while P. brevispinus and Pycnopodia inhabit both rocky and sandy substrates. One Pycnopodia, one P. giganteus, and two Kelletia were placed in a fresh circulating seawater tank measuring 90cm x 90cm x 60cm (water level maintained at 25cm height). One Kelletia was placed with two Cancer in a separate tank with the same measurements. The snails were allowed to move freely in the tank along with the two sea stars and the crab. Observations were recorded for five days in each of the tanks. The escape response to P. giganteus was studied in the field during two separate SCUBA dives, in which the tube feet of P. giganteus were touched to the foot of Kelletia. Results Population Studies The average density of Kelletia at random sampling locations within the Hopkins Marine Life Refuge was 1.62 per 5 m2 (SD=2.29, Stevenson: Natural History of Kelletia n=21), or 0.32 m-2. The depths at which the snails were found ranged from 5.5 m to 12 m, with the majority of snails deeper than 7.5 m. The snails were found on both rocks and sand. Rock locations included rocks ranging in size from small fist-sized rocks to large boulders, and sand beds included bare sand, mats of the tubiculous polychaete, Diopatra ornata, and sand channels between rock outcroppings. The mean length of snails found on the sand was 92.9 mm (SD=12.0, n=15), while the mean for rock locations was 78.4 mm (SD=14.8, n=26, Fig. 2). The size difference between the two populations was significant (t-test with unequal variances; pæ0.01). Lab Activity Patterns The snails in the lab characteristically buried themselves for extended periods of time, punctuated with bursts of activity in which the snails would move around the tank (Fig. 3). The snails ranged in size from 79 mm to 97 mm, and all snails buried themselves completely in the substrate at least once during the observation periods. When burying, the front edge of the foot dug into the sand and pulled sand up over the front edge of the foot. The siphonal canal was pulled completely under the surface of the substrate, and the snail then rocked the shell in a spiraling motion until burial was complete. Once burrowing began, all snails were buried within 15 minutes. The siphon was extended out of the sand only once out of 25 observed burial events. Activity patterns varied substantially among individual snails. Several remained buried for a very long time and became active only Stevenson: Natural History of Kelletia once or twice during the trial (Snail 10, Fig. 3c). Others buried themselves up to 5 times with intervening active periods of varying lengths (Snail 7, Fig. 3b). No diurnal pattern of activity was observed. On one occasion snail 9 followed the path of snail 11 over a distance of 195 cm, and snail 10 followed the path of snail 12 over a distance of 50 cm. In both instances, the 2 snails burrowed back into the sand adjacent to each other. The length and interval of buried and active periods were determined for each snail in each of the three sessions (Fig. 3). The median percent of time spent buried for all the snails was 84% (SD=21; Range: 34%-97%). The median length of buried periods was 28.6 hours (SD=40.1; Range: 8-152 hours). The median length of active periods in all three trials was 6 hours (SD=4.3; Range: 2-16 hours). The median movement rate during active periods in the lab was 16.5 cm per hour (mean-25.1 cm/hr; SD=21; Range: 21-79 cm per hour). There was no significant correlation between snail sizes and percent time buried, length of buried period, length of active period, or movement rate (p»0.16 for all regressions; Figs. 4-7). Field Activity Patterns Eight of the fifteen snails from the first tag-and-release trial were found 18.5 hours later. Of these, 5 snails were buried in the sand within 1 m of the release point. The other 3 snails had moved between 622 and 1087 cm and still appeared to be moving. All three snails were still on sand and had moved in the same direction. In the second group, 19 of the 20 snails were found within 1.5 hours, 16 were found after 4 hours, and 12 relocated after 73 hours. Stevenson: Natural History of Kelletia Nine snails were found either half-buried or completely buried at the first two observation times, while 6 snails were found buried in similar positions after 73 hours. Three of the 12 snails recovered after 73 hours had moved onto rocks from the sand, one of which was associated with a P. giganteus. Neither the snail nor the sea star were feeding. The 3 snails from the first tagged group and the 8 snails from the second tagged group that exhibited marked movement were used to calculate the field movement rate. The median movement rate in the field was 40 cm per hour (SD=18.1; Range: 11-87 cm per hour). This rate was not significantly different from laboratory estimates (t- test assuming unequal variances; p=0.31). Approximately two weeks after release, 3 snails from the first group of tagged snails were observed at least 40 meters away from the original release point (Location 1, Fig. 1). These three snails were at a mating aggregation (Location 2, Fig. 1). Gut content analysis and feeding Only one of five snails had food in its gut. The contents could not be identified. This snail was collected from the sand but was not buried. The other four snails, two collected from the sand and two from the rock, all had empty guts. The gut content analysis was thus inconclusive. However, four of the five snails were reproductively ripe. The fifth snail lacked a gonad and the inner surface of the shell was encrusted in the area where the gonad would have been. During the activity studies, Kelletia fed on the fish placed in the tank on two occasions: one snail sat on the fish for a period of hours, Stevenson: Natural History of Kelletia then moved off and buried itself. In the other case, evidence of rasping was found on the underside of the fish, indicating that a buried snail had fed on it from below, or a snail had extended its proboscis underneath the fish. In the first trial, three of the four snails came out of the substrate within 2 hours after introduction of the fish. The snails in the second tank did not react as quickly, though, and it was at least 21 hours before any snail came out of the substrate. In Trial 3, snails 9 and 11 (Fig. 3c) passed within 5 cm of the fish, but did not come in contact or stop to feed. Two T. brunnea, 2 T. pulligo, 3 T. montereyi, and 2 Calliostoma ligatum were consumed during both lab feeding trials. During capture, the Kelletia encountered the Tegula from different directions each time. Kelletia then forced its foot under the shell rim of the prey and gradually wrapped its foot around the underside of the Tegula. During feeding itself, the Kelletia rested on the back or side of its shell on the bottom of the tank. Complete consumption of prey lasted at least 6 hours. In two of the three instances of predation on T. montereyi, the head and foot were not consumed. During the second feeding trial using a new group of Kelletia (n=6), one T. montereyi was consumed completely. When offered other invertebrates, Kelletia only consumed the live sipunculid worm after it had crawled out of the test tube. In no case was a Kelletia observed inserting its proboscis into the test tubes to reach the invertebrate inside. In the field, Kelletia were found feeding on Tegula brunnea on three separate occasions. In one instance, a Kelletia was found in a Diopatra mat with its proboscis extended at least 3 times the snail’s 10 Stevenson: Natural History of Kelletia length, presumably feeding. The prey, however, could not be determined. Mating Aggregation Observations The density of snails in the mating aggregation (Location 2, Fig. 1) was 260 snails m-2. The mean size of the snails observed in the aggregation was 89.9 mm (SD=8.3; n=68; Range: 74-115 mm; Fig. 8). The mean size of Kelletia outside the aggregation (on sand and rock) was 84.4 mm (n=68; SD=15.0; Range: 36-122). The difference in snail sizes between the two populations was significant (t-test with unequal variances; pæ0.01). Escape Response Studies Kelletia exhibited no escape response to Pycnopodia or P. brevispinus in the laboratory. On three occasions Pycnopodia climbed over the snail, its tube feet making contact with the snail's foot; in each instance, the snail showed no reaction. On one other occasion, the P. brevispinus passed one arm over a Kelletia, also coming in contact with the snail’s foot. Again, the snail showed no escape response to the sea star. In the field, P. giganteus was placed in contact with a snail on two occasions. The snails showed no response either time. In addition, one tagged Kelletia was found underneath a P. giganteus, although the sea star was not feeding on it. The snail was not obviously trying to escape. Kelletia placed in the tank with the crab Cancer antennarius also did not exhibit escape responses. The Cancer succeeded in breaking off a few shards from the lip of the shell, but never actually 11 Stevenson: Natural History of Kelletia ate the snail. While being manipulated by the Cancer, the snail did withdraw into its shell and remained there for some time after manipulation. Discussion The depth distribution of Kelletia kelletii in southern California ranged from 2 to 40 m (Rosenthal, 1971). This contrasts with Monterey, where 5.5 m was the shallowest depth at which K. kelletii was found. Searches at shallower depths yielded no Kelletia. Further detailed examination of the subtidal zone in the Hopkins Marine Life Refuge may be necessary to determine the validity of the observed difference in the upper depth limits. Areas outside the kelp bed deeper than 12 m were not sampled, so a comparison between the deeper limits is not possible at this time. The large standard deviation associated with the mean density of snails indicates that Kelletia has an aggregated distribution throughout the kelp forest. Densities of K. kelletii in two southern California sites were 1.82 m-2 and 0.73 m-2 (Rosenthal,1971), compared to the 0.32 m-2 observed in the kelp bed in Monterey This could be related to the recent range extension of K. kelletii into the area, and the local population has not attained the densities observed in southern California. Since my study was conducted during Kelletia’s reproductive season, another possibility is that many snails were concentrated at the mating aggregation, artificially decreasing the density estimates. The burial behavior of the snail may also have lowered the estimated density, since it was not known at the time of field sampling that adult snails bury themselves. 12 Stevenson: Natural History of Kelletia Further sampling including inspections of sand patches for buried K. kelletii are necessary to determine the percent of the population buried at any given time. No snails smaller than 35 mm were found. In southern California, juveniles as small as 18 mm were found (Rosenthal, 1971) Further investigation is required to determine if juvenile snails have a different distribution than adults in Monterey. The significant difference in snail sizes on rock and on sand could indicate a change that favors moving from the rock to the sand as they grow older. The cues for such a shift are unknown, although one possibility could be a change in diet. It is also possible that, since Kelletia are quite mobile snails, the observed differences in snail sizes between sand and rock habitats are coincidental. Further sampling and larger sample sizes could clarify this issue. Lab and Field Activity Patterns Only Kelletia smaller than 40 mm in length bury themselves in the substrate in southern California (Rosenthal, 1971). In Monterey Bay, adult snails (79-97 mm) buried themselves both in the lab and in the field. The reason for this difference is not known. Activity patterns may be associated with foraging behavior. Due to the large proportion of empty stomachs, gut studies did not reveal whether Kelletia forage while buried or while active. However, several indirect lines of evidence suggest foraging occurs during active periods rather than when buried. The Kelletia fed on prey that they would only encounter while active, namely the Tegula. In addition, when food was added to the the activity pattern 13 Stevenson: Natural History of Kelletia tank in Trial 1, three of the four snails emerged within 2 hours and moved around on the sand, rather than burrowing toward the food. Possession of such a long, extensible proboscis would allow Kelletia to feed on buried prey without expending the energy required to burrow. The snail observed in Diopatra with its proboscis extended was feeding in this manner. The snail in Trial 2 may have done likewise to feed on the fish from below. Finally, snails were stationary while buried in the tanks, suggesting they were not actively seeking food. Why the burial occurs is unclear. Burial may be a defense against predation. Juvenile Kelletia bury themselves to avoid contact with P. giganteus, although they do not bury in direct response to contact with the sea star (Rosenthal, 1971). The decreasing trends of percent time buried and average length of burial interval with increasing size (Figs. 4, 5) may also support a defensive function for burial, since larger individuals are often less susceptible to predation (Paine, 1976). Further sampling is required to confirm these observed trends. Alternatively, burial may be unrelated to predation. Snails buried themselves in the complete absence of predators and showed no escape responses to any of the predators tested. Lack of an escape response is typical of southern California individuals as well (Rosenthal, 1971). Although P. giganteus has been observed to eat Kelletia, the lack of an escape response is not surprising since a trophic association (i.e. communal feeding on the same prey item) between these two species has been described (Rosenthal, 1971) Kelletia associate with two other sea stars, Pisaster brevispinus and 14 Stevenson: Natural History of Kelletia Dermasterias imbricata (Rosenthal, 1971). It is possible that, for similar reasons, the snail does not exhibit an escape response to P. brevispinus or Pycnopodia helianthoides, although no association between Pycnopodia and Kelletia has been reported. In addition, the shell is quite massive in the larger adults, and makes it difficult for Cancer to break open the shell in order to consume the snail. The burial period may also be a period of inactivity after foraging since little or no movement was observed while the snails were buried. A study conducted on P. giganteus indicates a similar pattern of inactivity after feeding (Harrold, 1981). Activity patterns may also be related to the reproductive cycle of the snail. The active periods of snails in the lab were characterized mainly by movement in straight lines as indicated by diatom film trails, covering large distances until an obstacle was encountered (such as a tank wall). This movement could be a search for unknown cues indicating the mating aggregation. The snails that followed each other in Trial 3 along very similar trails in the activity pattern tanks indicate the snails may be responding to a mating cue. The cue may be in the mucus secreted by the snails; Rosenthal (1970) speculates that this may be the case as well. Future characterization of the mucus would determine the validity of this idea. There may also be a behavioral hierarchy of activity and foraging, depending on the reproductive condition. During non¬ mating times of the year, activity patterns of the snail may be determined by foraging needs. Once mating cues have occurred, the activity pattern may be governed mainly by searching for mates. 15 Stevenson: Natural History of Kelletia During this time, feeding may become secondary. Two lines of indirect evidence support this idea. The four snails with empty guts had very well-developed gonads and were active (rather than buried) when collected. In addition, in the lab five Kelletia with Tegula available for consumption spent all their time mating and laying eggs, suggesting that mating had taken a priority over feeding. Observations at non-mating times of the year would clarify the possibility of such a hierarchy of behavior. Mating Aggregation Observations The larger mean size of snails within the aggregation than outside the aggregation indicates that only sexually mature (represented by larger) individuals are present in the mating aggregation. No snail inside the aggregation was smaller than 70 mm (Fig. 8). This can be approximated as the sexual maturity threshold for K. kelletii. This data is roughly consistent with data from southern California, which show the smallest snail in a mating aggregation to be 62 mm in length (Rosenthal, 1970). Whatever cues may be in effect for the mating aggregation work over a large distance and seem fairly specific considering three snails from the original tagged group were observed withing meters of each other. However, the cues remain undescribed and identifying them would be a very interesting avenue of research. The activity patterns of all the snails at non-mating times of the year would be very informative as well. 16 Stevenson: Natural History of Kelletia Acknowledgements I would like to extend a heartfelt thanks to Dr. Jim Watanabe of Hopkins Marine Station for the invaluable assistance provided, without which this project would have been completely unmanageable. Advice on subtidal methods and help in improving this paper are greatly appreciated. Thanks also to Gary Villa of Powers Lab at the Hopkins Marine Station for allowing me to convince him to spend more time underwater than is probably healthy for a human being. Finally, I would like to thank all the people at the Marine Station who made my three month stay there one of the most memorable events of my life. Stevenson: Natural History of Kelletia Literature Cited Harrold, C. 1981. Feeding ecology of the asteroid Pisaster giganteus in a kelp forest system: Prey selection, predator-prey interactions, and energetics. Ph. D. dissertation, University of California Santa Cruz. Herrlinger, T. J. 1981. Range extension of Kelletia kelletii. The Veliger 24 (1): 78. Morris, R. H., Abbott, D. P., Haderlie, E. C. (1980). Intertidal Invertebrates of California. Stanford University: Stanford University Press. Paine, R. T. 1976. Size-limited predation: an observational and experimental approach with the Mytilus-Pisaster interaction. Ecology 57: 858-873. Rosenthal, R. J. 1969. A method of tagging mollusks underwater. The Veliger 11 (3): 288-289. Rosenthal, R. J. 1970. Observations on the reproductive biology of the Kellet's whelk, Kelletia kelletii (Gastropoda: Neptuneidae). The Veliger 12: 319-324. Rosenthal, R. J. 1971. Trophic interaction between the sea star Pisaster giganteus and the gastropod Kelletia kelletii. Fishery Bulletin 69 (3): 669-679. 18 Stevenson: Natural History of Kelletia Figure Legends Fig. 1: Map of the Kelp Forest and the Hopkins Marine Station of Stanford University in Pacific Grove, California. Location indicates the drop site for tagged snails, and Location 2 indicates the mating aggregation discovered on May 17. Fig. 2: Frequency histogram of snail size of K. kelletii found outside of the aggregation; on rock and on sand. Fig. 3: Graphs showing the burial intervals for three trials of observations of K. kelletii in the laboratory. 3a shows Trial 1 lasting 65 hours, 3b shows Trial 2 lasting 216 hours, and 3c shows Trial 3 lasting 74 hours. Note the scales are different between 3a & 3c and 3b. White rectangular boxes indicate burial periods with length in hours written in; plain horizontal bars indicate activity periods. The shaded vertical bars indicate periods of no observations. Fig. 4: Snail size versus percent time the snail spent buried, with the fitted regression line and regession equation displayed. Correlation is insignificant with a p value 50.05 Fig. 5: Snail size versus average length of burial period in hours with the fitted regression. Fig. 6: Snail size versus average length of active period in hours with fitted regression. 19 Stevenson: Natural History of Kelletia Fig. 7: Snail size versus average rate of movement in centimeters per hour with fitted regression. Fig. 8: Frequency histogram of size of snails found in mating aggregation. 191 2 Seesoven of C 3 0 14 Stevenson: Natural History of Kelletia Fig. 2 o statatatataavaaaaa- 2- Size (mm) 22 Fig. 3a Snail 1 28.6 Snail 2 28.6 Snail 3 35.35 Snail 4 - 22.225 Fig. 3b L Snail 5 - 71.5 Snail 6 — 2 1 L 51.5 Snail 7 1 Snail 8 - Fig. 30 Snail 9 Snail 10 Snail 11— Snail 12- Stevenson: Natural History of Kelletia Smelt Added 29.7 10 hours 23.3 78.5 151 33.25 30 hours 1 70.5 22.25 152.25 5 68 hours 5 . 23 1 — 0.9 0.8 0.7 9 0.6 - 0.5 2 0.4 0.3 0.2 0.1 75 Fig. 4 85 Snail Size (mm) 24 Stevenson: Natural History of Kelletia %time buried f (x) = -O.01x +1.66 100 100 90 80 70 E 60 50 40 k 30 20 10 75 80 Fig. 5 85 90 Snail Size (mm) 25 Stevenson: Natural History of Kelletia Avg. Burial Interval f (x) = -1.96x + 220.06 100 14 12 10 75 80 Fig. 6 85 Snail Size (mm) 26 Stevenson: Natural History of Kelletia Avg. Active Interval f (x) = 0.24x 14.48 95 100 80 70 60 50 § 40 90 20 10 75 80 Fig. 7 85 90 Snail Size (mm) Stevenson: Natural History of Kelletia E Avg. Moyt. Rate f (x) = -O.95X + 116.64 95 100 16 12 Stevenson: Natural History of Kelletia Fig. 8 HE aaa- ktatatatava- statatatatattaaa- 8 - Size (mm) 28