Andrus and Legard Intertidal Chiton Habitats page 10 avoidance of sunlight has been previously noted (Ricketts and Calvin, 1968). This species was rare intertidally, being more common subtidally. STENOPLAX HEATHIANA Stenoplax heathiana generally was found on the sides of large, rather bare rocks resting on a sandy bottom; this is in accord with observatinns by Johnson and Snook (1935) and Ricketts and Calvin (1968). There seemed to be no size¬ habitat relationship. One reason these animals can occur on a barren substrate is they eat large bits of drift algae that lodge at the base of the rocks (MacGinitie and MacGinitie, 1968). TONICELLA LINEATA Barnes (1972) notes that the presence of encrusting coralline algae is critical to the habitat of Tonicella lineata in the area he studied. Our observations support thisd T. lineata was rarely found without coralline algae and was most abundant in coralline-dominated tidepools or subtidally with corallines, both erecat forms and crustose about 2 mm thick. It additionally was found next to Strongylocentrotus purpuratus and among Anthopleura xanthogrammica. T. lineata was rarely exposed to direct surf, occurring primarily below the 0.0 ft tide level, though sometimes found at higher levels in tidepools. Point Pinos was the only site where T. lineata was found in crevices, in the open. Only one animal was found at Stillwater Cove. Andrus and Legard Intertidal Chiton Habitats page 11 JUVENILE CHITONS The habitat of the majority of juvenile chitons was: a) low in the intertidal ranging between the O and +3 foot tidal levels; b) damp; c) shady or dark; d) protected from wave shock by surrounding boulders, overhanging ledges, or by being within crevices. This zone corresponded to that of tunicates and sponges. Yet a young chiton was never found on either, but rather on bare rock or rock encrusted with such algae as Corallina gracilis forma densa Collins, and members of the genera Lithothamnion, Lithophyllum, and Hildenbrandia. SUMMARY 1. The habitats of 12 intertidal chitons from six sites on the Monterey Peninsula in central California picked for variability in surf exposure and substrate type, were described in terms of physical factors and biological associates. The chitons were Cryptochiton stelleri, Cyanoplax hartwegii, Ischnochiton regularis, Katharina tunicata, Mopalia ciliata, Mopalia hindsii, Mopalia lignosa Mopalia muscosa, Nuttallina californica, Placiphorella velata, Stenoplax heathiana, and Tonicella lineata. 2. The most important environmental parameters in determing chiton habitats proved to be surf strength, light exposure, substrate compositin, moisture and biological associates. Andrus and Legard Intertidal Chiton Habitats page 2 INTRODUCTION Chitons make up a conspicuous postion of the intertidal fauna of the California coastline. Various species are known to be characteristic of particular intertidal zones (Ricketts and Calvin, etc.), yet little is known about their specific habitats. Only two researchers have given this topic more than cursory treatment in print; Barnawell, (1954) for Mopalia spp. and Barnes (1972) for Tonicella lineata (Wood, 1815). We selected six areas of the Monterey Peninsula shore¬ line, differing in exposure and substrate, and examined the speific habitats of the following chiton species: Cryptochiton stelleri (Middendorff, 1846); Cyanoplax hartwegii (Carpenter, 1855); Ischnochiton regularis (Carpenter, 1855); Katharine tunicata (Wood, 1815); Mopalia ciliata (Sowerby, 1840); Mopalia hindsii (Reeve, 1847); Mopalia lignosa (Gould, 1846); Mopalia muscosa (Gould, 1846); Nuttallina californica (Reeve, 1847); Placiphorella velata Carpenter in Dall, 1878; Stenoplax heathiana (Berry, 1946); and Tonicella lineata. These habitats are herein described. Also included is a description of the habitat where juvenile chitons were most commonly found. MATERIALS AND METHODS From April 22 to May 9, 1974, each study site was surveyed during the lower lowtides. Descriptions of plant and animal page 3 Andrus and Legard Intertidal Chiton Habitats associates of each species of chiton were recorded, along with detailed chiton density maps (maps on file at Hopkins Marine Station, Pacific Grove, California). Species were collected and identified using Burghardt and Burghardt, 1969, the key to chitons in Light, et.al., (1964); and a preliminary copy of the chiton key appearing in Smith and Carlton (1975). From May 10 to May 28, all six study sites were examined for habitat parameters characteristic of the most abundant chiton species. Relative surf and sun exposure, substrate conditions and vertical distribution (as determined by algae distribution - Doty, 1946) were noted. Sites were examined at high tide as well as low water, to determine the surf strength. STUDY SITES Figure 1 shows the locations of the sites on the Monterey Peninsula. Yankee Point consists of large granite platforms split by many deep channels. Isolated shallow tidepools and deep pools, with sparse flora and abundant fauna, are exposed to strong surf action. Carmel Point consists of a broad belt of large granite boulders bordered by a sandy beach, it experiences moderate surf action. The boulders provide for few tidepools, and only a few small, protected channels are present. Stillwater Cove is protected by a natural off-shore Andrus and Legard Intertidal Chiton Habitats page 4 breakwater of rocks. The study site substratum consists mainly of horizontal tables of shale exposed at low tide. at the north edge of a sandy beach. The Cypress Point site resembles the site at Yankee Point, but the slope of the terrain is steeper and a large algae are more abundant. Point Pinos marks the south entrance to the Monterey Bay. Granite promotories and large, isolated rocks are exposed to strong surf. These formations partially protect the study area which was an area along the shoreline and was composed of boulders separated by channels and pools. Mussel Point is exposed at the south side of Monterey Bay. The granite substrate often forms vertical surfaces with distingt algal zonation. The data collected during our study are presented in table and figure form. Table 1 lists algal and animal associates, surf strength, vertical range, and additional comments useful in finding each species. DESCRIPTION OF HABITATS CHARACTERISTIC OE SPECIES STUDIED CRYPTOCHITON STELLERI Cryptochiton stelleri was found next to and in very deer tidepools and channels. They were not predominantly on huge rocks on sandy beaches, contrary to the findings of MacGinitie and MacGinitie (1968). We encountered individuals at the t1.O Andrus and Legard Intertidal Chiton Habitats page 5 ft. tidal level, totally exposed to full sunlight. This conflicts with reports that individulas are sensitive to light and only remain out of water when it is foggy (Ricketts and Calvin, 1968). CYANOPLAX HARTWEGII Cyanoplax hartwegii was found in two distinct habitats: 1) under Pelvetia fastigiata (J. G. Agardh) DeToni; and 2) in small, wet crevices or depressions. Occasionally individuals were found under Fucus distichus Linnaeus. Apparently, constant moisture and protection from direct surf are major characteristics of the C. hartwegii habitats. Less than 2% of those studied were exposed to direct surf, perhaps becasue they cannot maintain attachment when exposed to wave shock. Macroalga cover was not always present, though crustose coralline algae or Hildenbrandia occidentalis Setchell were consistent companions at all locations except the wet shale at Stillwater Cove. C. hartwegii's common occurance under fronds of Pelvetia is described by Ricketts and Calvin (1968), and we confirm this association. We found no avoidance of direct sunlight, as long as sufficient moisture was present (e.g., wet shale at Stillwater Cove), an observation in conflict with one recorded by Ricketts and Calvin (1968). ISCHNOCHITON REGULARIS Ischnochiton regularis was found only on the bottom of page 6 Andrus and Legard Intertidal Chiton Habitats smooth rocks, 15 cm to 30 cm in diameter, resting on a rocky bottom. The only other organisms found on these rocks were unidentified microscopic green and blue-green algae. Individuals were usually found in groups of two or three per rock. All seemed to avoid direct sunlight. No mention of I. regularis habitat was found in the literature. KATHARINA TUNICATA Katharina tunicata was abundant in two distinct habitats: 1) in low numbers at the Iridaea flaccida (Setchell and Gardner) Hollenberg and Abbott level of almost vertical granite surfaces, a habitat found at almost all the study sites; and 2) in higher numbers in shallow, exposed tidepools with crustose coralline algae covering the bottom; this habitat occurred only at Yankee and Cypress Points. In addition, small individuals (less than 6 cm long) were found in depressions inhabited by Strongylocentrotus purpuratus (Stimpson, 1857) and Anthopleura xanthogrammica (Brandt, 1835). At Yankee Point we observed a size gradient with larger animals further from the surf. The largest were found in the protected channels on the leeward walls. Our results agree with previous work (MacGinitie and MacGinitie, 1968) in that individuals were found along open, rocky coasts, not necessarily in direct surf, but not in a habitat as well protected as Stillwater Cove. Andrus and Legard Intertidal Chiton Habitats page 7 MOPALIA CILIATA Mopalia ciliata was the chiton least encountered, perhaps because it was inconspicuous. Tucked in crevices among Mytilus californianus Conrad, 1837, Tetraclita squamosa rubescens Darwin, 1854, crustose coralline algae, Pelvetia fastigiata and Endocladia muricata (Postels and Ruprecht) J. G. Agardh, members of this species often had its girdle buried in sand. Found mainly below the +2.5 ft tidal level and noted subtidally at Mussel Point, this animal was exposed to little or no surf. MOPALIA HINDSII We found Mopalia hindsii to be more exposed to air during low tides than the other Mopalia species. Individuals were usually in crevices nestled among associated algae, and often covered with a thick algal growth on the valves. MOPALIA LIGNOSA Mopalia lignosa has the most specific habitat of the chitons in this study. Individuals inhabited the undersides of movable rocks resting on rocky bottom. Some animals were found on the sides of rocks or in deep, smooth crevices and occasionally at the bottom of high tidepools. MOPALIA MUSCOSA Mopalia muscosa was found predominantly in two habitats: Andrus and Legard Intertidal Chiton Habitats page 8 1) in wet, protected, large crevices from the +1.5 ft to t3.0 ft. tidal levels; and 2) in high, protected tidepools up to +5.0 ft tidal level. In addition, after individuals were found under algae (Fucus distichus and Pelvetia fastigiata), and under rocks. At Stillwater Cove, a high density also was found on slight irregularities along flat, horizontal, very wet shale. None were exposed to direct surf. More than 97% of the individuals examined were in direct contact with moisture; either touching a pool of water (in tidepools or crevices). lying partially buried in moist sand, or sitting under wet macroalgae. More than 90% of the individuals examined had their anterior ends lower than their posterior ends and in contact with moisture. Almost all individuals (with the exception of those at Stillwater Cove) were found near either Peyssonnelia meridionalis Hollenberg and Abbott or crustose coralline algae. NUTTALLINA CALIFORNIA Nuttallina californica was found in three distinct situations. Listed in order of highest to lowest density. these were: 1) the bottom of bare rock depressions the size of their bodies; 2) squeezed between the bases of Tetraclita squamosa rubescens, Pollicipes polymerus, (Sowerby, 1833), and Mytilus californianus; and 3) wedged in small crevices in high, shallow tidepools. Rare individuals were found in large, shallow depressions at the base of Pelvetia fastigiata and Postelsia Andrus and Legard Intertidal Chiton Habitats page 9 palmaeformis Ruprecht. Surf strength at depression localities varied widely from very strong at Yankee Point to moderately weak at Stillwater Cove. Nuttallina californica was found in areas both with and without macroalgae, though highest densities were in the macroalgae-free depressions. Apparently. a major habitat factor is the need for protection from the direct shearing force of the surf. Only about 5% were not firmly entrenched in a crevice, depression, or space where the surf's force was depleted. MacGinitie and MacGinitie (1968) say N. californica inherit and live in deep depressions in rocks exposed at low tide, and feed on debris which collects in these depressions. We found individuals on rocks exposed to a wide range of surf strengths, but few such rocks were in areas of weak surf. More than 98% of the animals were in the presence of erect or crustoee coralline algae. None were found on flat areas, unless sessile animals or high¬ profile algae were present. The largest individuals were found in the high, shallow tidepools, but here the density was very low. PLACIPHORELLA VELATA Placiphorella velata were found on the flat sides of movable rocks and on the walls of deep tidepools, always below the -1.0 ft tidal level. The individuals were always found in shade and always with red crustose coralline algae. Their Andrus and Legard Intertidal Chiton Habitats page 13 ACKNOWLEDGMENTS The authors would like to thank the faculty and staff of Hopkins Marine Station, in particular Robin Burnett and Donald and Isabella Abbott. The description of juvenile chiton habitats was done entirely by Rich Gomez for which we thank him. Finally, special thanks to Chuck Baxter for his invaluable help as an advisor. Andrus and Legard Intertidal Chiton Habitats page 14 LITERATURE CITED Barnawell, Earl Baker 1954. The biology of the genus Mopalia in San Francisco Bay. M. A. Thesis. Dept. of Zoology, University of California, Berkeley. 85 pp.; 9 figs. Barnawell, Earl Baker 1960. Mopalia hindsii recurvans, subspec. nov. (Amphineura). The Beliger 3(2): 37-40; plt. 6. Barnes, James Ray 1972. Ecology and reproductive biology of Tonicella lineata (Wood, 1815). Ph.D. Dissertation, Dept. of Zoology, Oregon State University. 161 pp.; 47 figs. (June 1972) Burghardt, Glenn E. and Laura E. Burghardt 1969. A collector's guide to West Coast Chitons. 45 pp.: 4 plts. San Francisco, Calif. (San Francisco Aquarium Society, Inc.) Doty. Maxwell Stanford 1946. Critical tide factors that are corcelated with the vertical distribution of marine algae and other organisms along the Pacific coast. Ecology 27(4): 315-328; 6 figs. Johnson, Myrtle Elizabeth and Harry James Snook 1935. Seashore animals of the Pacific Coast. 659 pp.: 700 figs.; 11 plts. New York, N. Y. (The MacMillan Co.) Andrus and Legard Intertidal chiton habitats page 15 Light, Sol Felty 1964. Intertidal invertebrates of the central California coast. Revised by Ralph I. Smith, Frank A. Pitelka, Donald P. Abbott, and Frances M. Weesner. xiv + 446 pp.; 138 figs. Berkeley, Calif. (University of Calif. Press). MacGinitie, George Eber and Nettie MacGinitie 1968. Natural history of marine animals. xii + 523 pp.: 286 figs. New York, N.Y. (McGraw-Hill Book Co., Inc.) Ricketts, Edward F. and Jack Calvin 1968. Between Pacific tides. 4th ed. Revised by Joel W. Hedgpeth. xiv + 614 pp.; illus. Stanford, Calif. (Stanford University Press). Smith, Gilbert Morgan 1969. Marine algae of the Monterey Peninsula. 2nd ed. inc. 1966 Supplement by George J. Hollenber and Isabella A. Abbott. ix + 752 pp.; illus. Stanford, Calif. (Stanford University Press). Smith, Ralph I. and James T. Carlton, eds. 1975. Light's Manual: Intertidal invertebrates of the central California coast. Third edition. (University of California Press), Berkeley, Los Angeles, London. 716 pp., 156 plates. Stimson, John 1970. Territorial behavior of the owl limpet, Lottia Gigantea. Ecology 51(1): 113-118; illus. Andrus and Legard Intertidal Chiton Habitats TABLE LEGEND Table 1 List of chiton species with corresponding algal and animal associates, vertical range, average spring surf strength, and additional comments. page 16 Andrus and Legard — — O 9. o O 6 + OBoow — + poc. u — ooc HHH.8 d O + EBIO RI 0 0 0 1 0 10 m : 5 1O - — a + R H- 10 S OO Intertidal Chiton Habitats — So 0 1 J H. OIR OIO O POUBO oO - O S -O Bm 10 HO 9O —90 -0 0 O . 8 R ON C c H0 —. o BH. Os ss OOO H ON oo Om Th. SDOE ROm. 2O OODUBE — —OuD OU —0 H OO OOHOO S 0 m 0 : 5. uo ( — O 6 2 I + OO oc OROO 8o o 3 u OC B 5 Q H¬ OHO o O. 0 —30 0 38 e 3 o OH 2 ththo OO + O H. G C. o 8 OO . . 95 2 —OR OP O oBugu 1O 58 58 ou +h 9. oa- p965 OTOOE H 00 8 5 0 m O 165 o 212 19 0 0 O m . — 9 00 5 ojo 9 0 Ss 5 L6 o6 25 35 28 5 5c T 6 OOTO DORR O + + + + OUCOE uo O oho H- + +- + OS 5 85 —O 0 50 DOO oot Oo So 3 110 o. Ou 8 +. + C O O O HOO: O + +-0 ( 0 H. 5 5 6 3 OE Andrus and Legard Intertidal Chiton Habitats FIGURE LEGEND Figure Map of Monterey Peninsula, California showing the locations of the six study areas. firas t legar Tigune 1 Cypress Pt. Pt.ps us laeifie Gre ter Coe Carme e Vankee Pt. Montereg Bag S Maofereg 13 12 1 ale (oikes)